A total of 154 of Escherichia coli (E. coli) isolates were isolated from faecal specimens of healthy cattle (n=100). Serotyping of 154 E. coli isolates indicated that 112 (72.72%) isolates were typeable, 28 (18.18%) untypeable and 14 (9.09%) were rough. The most predominant serotype observed was O22 followed by O56, O60, O120 and O1. Antibiogram pattern using a total of 25 different antibiotics indicated that high resistant was present against clindamycin, metronidazole and penicillin followed by cephalothin, neomycin, kanamycin, cephalexin, streptomycin, furazolidone and tetracycline antibiotics. The amplification of tet(A) and tet(B) resistant determinants by PCR resulted in generation of 372bp and 228, respectively. The tet(A) gene was predominant gene compared to tet(B) gene. This study using pheno-genotypic characterization indicated the presence of antimicrobial resistant E. coliisolates amongst healthy cattle, emphasizing that effective strategy should be applied to persist the efficiency along with ideal usage of novel antibiotics though minimizing the risk of antibiotic resistant bacteria. arabic 11 English 82
Abdusalam Sharef Abdusalam Mahmoud(3-2020)

A serological survey was carried out in Libya to investigate the circulation of Rift Valley fever virus (RVFV) among domestic ruminants. A total of 857 serum samples were collected from year 2015 to 2016 in eleven provinces of Libya belonging to five branches of the country. Samples were tested for RVFV antibodies using a competitive EnzymeLinked Immunosorbent Assay (c-ELISA). Antibodies specific for RVFV were not detected in any of the 857 samples. However, a statistical analysis was carried out to assess the maximum expected number of infected animals and the maximum expected prevalence of RVFV among Libyan ruminants’ populations according to the sampled population. The overall maximum expected prevalence was estimated to be 1.8% for cattle and 0.4% for small ruminants. Results seem to exclude the circulation of RVFV, however, a surveillance plan should be implemented in areas at risk of RVFV introduction. arabic 10 English 55
Abdusalam Sharef Abdusalam Mahmoud, (6-2018)

Aims: To determine the seroprevalence of Toxoplasma gondii (T. gondii) in women from Aljalla Maternity and Gynaecology Hospital (AMGH) and to evaluate the association between the infection and potential risk factors. Methods: A cross-sectional study was conducted at AMGH in Tripoli during the year 2012. Data on potential risk factors were collected by a structured questionnaire and results of the test. The output data of questionnaire were used to assess potential risk factors for positive outcome. All sera (n = 500) was screened against ToxoG, by using the Vidas machine automated enzyme-linked florescent immunoassay. The association between the outcome variables and its potential risk factors were screened in a multivariate analysis. A p-value < 0.05 was considered to be significant. Results: The overall seroprevalence was estimated to be 50.8% with 95% confidence interval (CI) (46.42%–55.18%). The results revealed that T. gondii infection is highly prevalent, and eating habits (consuming raw meat and/or raw vegetables or fruits) were highly significant associated in multivariable analysis. Expectedly, a significantly [odds ratio (OR):1.712; p =
Abdusalam Sharef Abdusalam Mahmoud(1-2019)