The epidemiological patterns of Bluetongue (BT) in North Africa and Mediterranean Basin (MB) dramatically changed by emergence of subsequent episodes of novel bluetongue virus (BTV) serotypes with highly pathogenic indexes and socio-economic impacts. The objective of the study was to investigate the sero-prevalence and serotype distribution of BTV in Libya. During 2015-2016, a total of 826 serum samples were collected from domestic ruminants in Libya. All sera were assayed by competitive enzyme-linked immunosorbent assays (c-ELISA). C-Elisa-positive samples (43.3%; 173/400) were further analyzed by virus neutralization assay to identify BTV serotypes and determine the antibody titre of positive samples. An overall BTV sero-prevalence was 48.4% (95% CI: 45.0%-51.8%). Neutralizing antibodies were detected against the following BTV serotypes namely: BTV-1, BTV-2, BTV-3, BTV-4, BTV-9 and BTV-26. While BTV-1, BTV-2, BTV-4 and BTV-9 circulation was unsurprising as they have been responsible of the last year outbreaks in Northern African Countries, the detection of BTV-3 and BTV-26 was definitely new and concerning for the animal health of the countries facing the Mediterranean Basin. It is crucial that European and Northern African authorities collaborate in organizing common surveillance programmes to early detect novel strains or emerging serotypes in order to set up proper preventive measures, and, in case, develop specific vaccines and plan coordinated vaccination campaigns. arabic 13 English 96
Abdusalam Sharef Abdusalam Mahmoud(1-2018)

The Corona virus disease 19 (COVID-19) is a new global pandemic. World Health Organization (WHO) declared COVID-19 a public health emergency of international concern. Health Care Works (HCWs) are the primary sectors in contact with suspected patients. Thus, the knowledge, attitudes and practices of HCWs towards COVID-19 remain unclear. The objectives: To investigate the knowledge, attitudes and practices of Medical doctors about COVID-19 at Tripoli University Teaching Hospitals. Methods: This crosssectional survey was conducted from April 30 to 29 May, the week immediately after first case of COVID-19 reported in Libya. A pilot trail questionnaire was distributed and filled by taking the relevant information from medical staff doctors working at various medical and surgical units. The data coded according to variable and analysed by SPSS. Results: Of (250) participants, a total (100) of Medical staff doctors completed the surve\ (response rate 40%). The participants¶ qualification was (64%) MBBS, (14%) Master degree, (16%) PhD and (6%) professors. the mean age 35 years. (42%) were male and (58%) female participants. Most of the medical staff doctors (70%) were GPs, (22%) were specialists, (6%) were internship doctors and (2%) were consultants. Questionnaire survey revealed that (18%) of the respondents reported working experience of >20 years. The survey was considered if the Medical doctor in frontline healthcare workers (FLHCWs), our results demonstrate that, only 30% of medical staff doctors was provided care of COVID-19 patients while 70% was not in the FLHCWs. Most participants (79%) reported that no specific treatment of COVID-19. A significant proportion of medical doctors (68%) had good knowledge of the transmission, diagnostic method & prevention of COVID-19. According to the case report definition of WHO and ECDC, the survey results showed (69%) of the respondents reported that, the test should be repeated if the first RT-PCR results were negative from the patient represent the typical clinical symptoms of COVID-19. Our questionnaire survey revealed that (68%) of the medical staff doctors¶ participants agree that wearing face masks prevent the infection while (32%) reported that wearing face masks could not prevent the infection. Overall, Medical staff doctors in Tripoli University Teaching Hospital showed expected level of knowledge and attitudes about COVID-19. The findings survey suggests that due to the limited medical staff doctor¶s representative, it must be cautious when generalizing these findings to other medical doctors residing in other regions of the country. As intentional threat of COVID-19 continuous to emerge, the results survey highlights the importance of continuous health educational programs from the government and national health authorities that well improve and updated knowledge of the HCWs regarding COVID-19, which also result in increasing their attitudes and practices towards COVID-19. Further studies are warranted to confirm our findings. arabic 17 English 99
ِAbdusalam Sharef (12-2020)

A total of 154 of Escherichia coli (E. coli) isolates were isolated from faecal specimens of healthy cattle (n=100). Serotyping of 154 E. coli isolates indicated that 112 (72.72%) isolates were typeable, 28 (18.18%) untypeable and 14 (9.09%) were rough. The most predominant serotype observed was O22 followed by O56, O60, O120 and O1. Antibiogram pattern using a total of 25 different antibiotics indicated that high resistant was present against clindamycin, metronidazole and penicillin followed by cephalothin, neomycin, kanamycin, cephalexin, streptomycin, furazolidone and tetracycline antibiotics. The amplification of tet(A) and tet(B) resistant determinants by PCR resulted in generation of 372bp and 228, respectively. The tet(A) gene was predominant gene compared to tet(B) gene. This study using pheno-genotypic characterization indicated the presence of antimicrobial resistant E. coliisolates amongst healthy cattle, emphasizing that effective strategy should be applied to persist the efficiency along with ideal usage of novel antibiotics though minimizing the risk of antibiotic resistant bacteria. arabic 11 English 82
Abdusalam Sharef Abdusalam Mahmoud(3-2020)