This study assessed the biodistribution of autologous leucocytes radiolabelled with technetium-99m stannous fluoride colloid (99mTcSnC) for detection of foci of induced inflammation in dogs. Venous blood was collected from seven healthy dogs and incubated with 99mTcSnC for 1 h at room temperature. Radiolabelled samples were injected intravenously (IV) and the dogs were scanned using a gamma camera. Another seven healthy dogs were injected intradermally with tumour necrosis factor α and then IV with 99mTcSnC radiolabelled autologous blood 3 h later before being scanned. The radiolabelled leucocytes localised to sites of inflammation by 30 min post-injection. IV injection of autologous leucocytes radiolabelled with 99mTcSnC appears to be a sensitive method for localisation of induced foci of inflammation in dogs.
Mohamed Hamrouni S. Abushhiwa, Nouria S.Salehi, Meir Lichtenstein, Peter M. Lording, Peter J. Finnin, Robert C. Whitton, Jennifer A. Charles, Bruce W. Parry (8-2010)

Objective: Metacarpophalangeal joint (MCPJ) radiographic parameters were developed to evaluate MCPJ conformation of equine forelimbs, but the effect of joint rotation during radiography on these parameters has not been reported. The current study examined the effect of the rotation around the vertical axis of MCPJ on 27 MCPJ parameters (12 angles and 15 ratios) and identified the acceptable range of rotation at which minimal amount of changes occurred in these parameters. Design: Descriptive study Animals: MCPJs of seven right forelimbs from seven different horses with no MCPJ abnormalities. Procedures: MCPJs of seven right equine forelimbs were loaded in a load cell to obtain the normal MCPJ angle. They were frozen at −20C° and then scanned using a CT-scan machine. The collected slices were reconstructed to obtain a 3D model of MCPJs, and the dorsopalmar view at zero angle (ZDP) were identified. The view was then rotated around the vertical axis of MCPJ. Snapshots were taken for MCPJ at −10°, −5°, Zero, +5° and +10° angles. All the parameters were measured on the total 35 radiographs and statistically analysed. Differences were estimated per 5° of MCPJ rotation for all the parameters. Results:Generally, the ratio parameters were less affected than the angular parameters during MCPJ rotation. Conclusion and clinical relevance: Rotational angles of vertical ±10° from ZDP were considered as an acceptable range for reliable radiographic measurements of MCPJ parameters. Keywords Equine; fetlock; morphometry; radiology; rotation
Abdulrhman Mohamed Salah Alrtib, Aiman Hussein Saleh Oheida, Mohamed Hamrouni S. Abushhiwa, Helen M.S. Davies(12-2021)

Animals alter their locomotor mechanics to adapt to a loss of limb function. To better understand their compensatory mechanisms, this study evaluated the changes in the fore-aft ground forces to forelimb lameness and tested the hypothesis that dogs unload the affected limb by producing a nose-up pitching moment via the exertion of a net-propulsive force when the lame limb is on the ground. Seven healthy Beagles walked and trotted at steady speed on an instrumented treadmill while horizontal force data were collected before and after a moderate lameness was induced. Peak, mean and summed braking and propulsive forces as well as the duration each force was exerted and the time to reach maximum force were evaluated for both the sound and the lame condition. Compared with the sound condition, a net-propulsive force was produced by the lame diagonal limbs due to a reduced braking force in the affected forelimb and an increased propulsive force in the contralateral hindlimb when the dogs walked and trotted. To regain pitch stability and ensure steady speed for a given locomotor cycle, the dogs produced a net-braking force when the sound diagonal limbs were on the ground by exerting greater braking forces in both limbs during walking and additionally reducing the propulsive force in the hindlimb during trotting. Consistent with the proposed mechanism, dogs maximize their double support phases when walking. Likely associated with the fore-aft force adaptations to lameness are changes in muscle recruitment that potentially result in short- and long-term effects on the limb and trunk muscles. arabic 13 English 78
Jalal Mohamed Abdelhadi(12-2012)