The detection of somatic mutations in tumours is essential for the understanding of cancer development and targeting therapy. The screening for BRAF V600E mutation is employed in clinical practice in Libya for its prognostic and potentially predictive role in patients with metastatic colorectal carcinoma (mCRC). Using of BRAF mutant DNA and wild type DNA targets, we found that the sensitivity of allelic discrimination-Real Time PCR was applicable. The Real Time PCR assay displayed increased analytical sensitivity in detecting the BRAF V600E mutation. The association of BRAF mutations with clinical and pathological features was assessed using Real Time PCR assay. Qiagen Real Time PCR Platform was utilised using a set of primers. forward 5-GAC. CTC. ACA. GTA. AAA. ATA. GGT. G 3, reverse 5-TCC. AGA. CAA. CTG. TTC. AAA. CTG. A. 3. Our study indicates that Real Time PCR-based assays is convenient to detect the BRAF V600E mutation in CRC and that BRAF mutations screening should not be restricted to selected patients on the basis of the clinicalpathological characteristics. arabic 9 English 63
Abdul M Gbaj, Abdulla Bashein(1-2018)

Lipid biochemistry can seem overwhelming, which is why it needs to be explained in a simple and straightforward manner. Ashour Saleh Eljamil, a renowned professor of biochemistry, has written this textbook for undergraduate students in the medical sciences, but its a resource that anyone can use to bolster their knowledge about this important subject. To fully understand biochemistry, you need to know how biomolecules are structured, which is why the first chapter emphasizes the individual chemical structure of various lipid classes. Youll also learn how dietary lipids are digested and absorbed as well as how their metabolism works in separate chapters focusing on fatty acids synthesis, fatty acid oxidation, acylglycerols and sphingolipids, glycolipids, cholesterol, plasma lipoproteins, steroid hormones, and fat-soluble vitamins. While scientists have studied lipid biochemistry for three centuries, its only in the past few decades that weve begun to understand why its so important. Gain a clearer understanding of the world with insights about bile acids, sterols, carotenoids, sex hormones, vitamin K and much, much more when you dive into the world of Lipid Biochemistry. arabic 5 English 35
Ashour Eljamil(3-2015)

Acinetobacter baumannii is an opportunistic pathogen causing various nosocomial infections. The aim of this study was to characterize the molecular support of carbapenem-resistant A. baumannii clinical isolates recovered from four hospitals in Tripoli, Libya. Bacterial isolates were identified and antibiotic susceptibility testing was per-formed using automated system. Carbapenem resistance determinants were studied phenotypically using two dif-ferent techniques: E-test; chromogenic culture media. Polymerase chain reaction (PCR) amplification was used to determine the presence of bla OXA23 and blaOXA51 genes among isolates. A total of 119 isolates were characterized, overall the resistance prevalence was extremely high for aminoglycosides (79-96.6%), fluoroquinolones (94-96%), cephalosporins (96.6-100%) and carbapenemes (93.2-100%), all isolates were susceptible to colistin. In addition, 97.5% of isolates were identified as multidrug resistance (MDR). Varying degree of phenotypic detection of car-bapenemes was determined; highest levels of carbapenemes were detected using chromogenic media (76.5%) com-pared with E-test (45.4 %). The carbapenem resistance-encoding genes detected were blaOXA23 (84%) and blaOXA51 (73.1%); the highest occurrence of blaOXA23 was demonstrated in Tripoli’s Central Hospital (5/5; 100%) then in Tripoli Medical Center (44/51; 86.27%). The co-occurrence of these genes was demonstrated in (75/119; 63%) showing dissemination of carbapenemes resistance MDR A. baumannii in hospitals. This study shows that the high prevalence of OXA-23 contribute to antibiotic resistance in … arabic 14 English 113
Nada Elgrew, Abdulla Bashein(1-2016)