The GC-box is an important transcriptional regulatory element present in the promoters of many mammalian genes, and is found in most, if not all, oxidative phosphorylation (OXPHOS) promoters. In the present study we examine the effects of three Sp1 family members (Sp2, Sp3, and Sp4) on the adenine nucleotide translocase 2, cytochrome c1, F1-ATPase β-subunit, and the mitochondria transcription factor (mtTFA) promoters in Drosophila SL2 cell line. Sp3, like Sp1, strongly activates transcription all four promoters. SP4 stimulates, moderately, but Sp2 had no effect. In addition, Sp3 can, like Sp1, inhibit transcription from the proximal promoter of the ANT2 gene through binding to the Cbox GC element. By contrast, Sp4 and Sp2 do not repress promoter activity. Furthermore, since Sp4 and Sp2 bind to the Cbox repression element on the ANT2 promoter, but do not repress transcription, inhibition of transcription … arabic 21 English 97
Ahmed Zaid(1-2012)

Acinetobacter baumannii is an opportunistic pathogen causing various nosocomial infections. The aim of this study was to characterize the molecular support of carbapenem-resistant A. baumannii clinical isolates recovered from four hospitals in Tripoli, Libya. Bacterial isolates were identified and antibiotic susceptibility testing was per-formed using automated system. Carbapenem resistance determinants were studied phenotypically using two dif-ferent techniques: E-test; chromogenic culture media. Polymerase chain reaction (PCR) amplification was used to determine the presence of bla OXA23 and blaOXA51 genes among isolates. A total of 119 isolates were characterized, overall the resistance prevalence was extremely high for aminoglycosides (79-96.6%), fluoroquinolones (94-96%), cephalosporins (96.6-100%) and carbapenemes (93.2-100%), all isolates were susceptible to colistin. In addition, 97.5% of isolates were identified as multidrug resistance (MDR). Varying degree of phenotypic detection of car-bapenemes was determined; highest levels of carbapenemes were detected using chromogenic media (76.5%) com-pared with E-test (45.4 %). The carbapenem resistance-encoding genes detected were blaOXA23 (84%) and blaOXA51 (73.1%); the highest occurrence of blaOXA23 was demonstrated in Tripoli’s Central Hospital (5/5; 100%) then in Tripoli Medical Center (44/51; 86.27%). The co-occurrence of these genes was demonstrated in (75/119; 63%) showing dissemination of carbapenemes resistance MDR A. baumannii in hospitals. This study shows that the high prevalence of OXA-23 contribute to antibiotic resistance in … arabic 14 English 113
Nada Elgrew, Abdulla Bashein(1-2016)

Objective Healthcare workers, particularly those working in departments that provide care for patients with coronavirus disease 2019 (COVID-19), are at a higher risk of this contagious disease than those who work in other departments. The aim of this study was to assess the psychological status of healthcare workers during the COVID-19 outbreak, which has compounded Libya's existing civil war-related problems. Methods A multi-center cross-sectional survey on depressive symptoms, anxiety symptoms, and abuse was conducted. The Hospital Anxiety and Depression Scale (HADS) was used to measure the prevalence of anxiety and depressive symptoms among healthcare workers. Results The data of 745 eligible healthcare workers from 15 hospitals were analyzed. Depressive and anxiety symptoms were compared to the basic characteristics of the participants to determine the association. A total of 420 (56.3 … arabic 17 English 93
Muhammed Elhadi, Ahmed Zaid(1-2020)