The most common preparation of tocotrienols is the tocotrienol-rich fraction (TRF). This study aimed to investigate whether TRF induced liver Nrf2 nuclear translocation and influenced the expression of Nrf2-regulated genes. Methods: In the Nrf2 induction study, mice were divided into control, 2000 mg/kg TRF and diethyl maleate treated groups. After acute treatment, mice were sacrificed at specific time points. Liver nuclear extracts were prepared and Nrf2 nuclear translocation was detected through Western blotting. To determine the effect of increasing doses of TRF on the extent of liver nuclear Nrf2 translocation and its implication on the expression levels of several Nrf2-regulated genes, mice were divided into 5 groups (control, 200, 500 and 1000 mg/kg TRF, and butylated hydroxyanisole-treated groups). After 14 days, mice were sacrificed and liver RNA extracted for qPCR assay. Results: 2000 mg/kg TRF administration initiated Nrf2 nuclear translocation within 30 min, reached maximum level around 1 h and dropped to half-maximal levels by 24 h. Incremental doses of TRF resulted in dose-dependent increases in liver Nrf2 nuclear levels, along with concomitant dose-dependent increases in the expressions of Nrf2-regulated genes. Conclusion: TRF activated the liver Nrf2 pathway resulting in increased expression of Nrf2-regulated cytoprotective genes.
Ahmed Elbadri M Atia(9-2020)

Background and Objectives: Compliance of community pharmacies with the proper practice of storage and dispensing of medicines is crucial for ensuring the quality and safety of medicines, particularly with the circumstance of recurrent electricity blackout in Libya. This study was aimed to assess the compliance of community pharmacies with the proper practice of drug storage in Tripoli city, Libya. Materials and Methods: A cross-sectional survey conducted in November 2020 targeting a total of 56 community pharmacies in Tripoli, Libya. The questionnaire was adapted from the WHO Checklist for Good Storage Practices and included 41 questions organized under five sections: socio-demographics, pharmacist’s attitude toward the quality of storage practices, queries on environmental storage conditions, the quality of facilities in the community pharmacy, and queries on storage and pharmacy practices. Data were presented as descriptive statistics. Results: Out of the total 56 visited pharmacies, a total of 46 (82.1%) pharmacists participated in the study with one pharmacist being interviewed in every pharmacy. Results showed that 15% of employees reported variety of cleanliness regulatory depending on cutting off running water circumstances in Tripoli and other reasons. About 78.8% participants observed dust in shelves and over the drugs packaging, and 22% of the participant pharmacies’ drugs exposure to direct sunlight. Additionally, 72% of employee experienced high temperatures in the pharmacy during electricity blackouts, whereas only 48% of them experienced humidity. In addition, 91.4% had alternative source of electricity, 44.3% had a power backup connected to the refrigerators. Conclusion: The compliance of majority of the community pharmacies operated in Tripoli is below standard. There is still need to improve the storage practices in the community pharmacies by obeying with the regulatory standards as specified by the Drug Regulatory Authority of Libya.
Ahmed Elbadri M Atia(5-2021)

Background: Management of partially edentulous patients with hard and soft tissue undercuts is complicated, and the use of flexible dentures for these cases offers dual advantages of aesthetics and flexibility. However, research shows a varying preference for flexible dentures among general dental practitioners, prosthodontists, and other dental specialties. Objectives: The aim of this study was to survey the attitude and awareness of a sample of Libyan dental clinicians towards advantages, disadvantages, indications, and contraindications of flexible dentures. Material and methods: A descriptive cross-sectional study was conducted among 250 dentists currently practicing in different cities of Libya. Only 218 dentists responded. Using Google form software, a self-administrable e-questionnaire consisting of thirty-one multiple choice questions with "yes," "no" or "not sure" answers encompassing major aspects of flexible dentures was conducted through an online survey. Results: Out of 250 Libyan dentists to whom the questionnaire was sent, only 218 dentists responded and our results revealed that the respondents were females more than males with a ratio (2:1) and more than half of them were of age group (35-60 years) work in governmental dental practice with more than ten years of experience and 34.9% of them prefer and often offer flexible dentures to their patients. The majority of respondents with a long-term success of the flexible denture were prosthodontists who had more than ten years of experience with a correct response rate of more than 50% of questions that reflected their attitude and knowledge. Conclusions: Despite the fact that flexible dentures are not taught in Libyan universities of dentistry, about more than one-third of respondents prefer and recommend this treatment to their patients, and the long-term success of these prostheses was dependent on clinicians' education and their clinical experience.
Yusra Muftah Saad Elfaidy, Warida Abdalla Elnaihoum, Aseidah Abdullah Elgotrany(4-2022)