The most common preparation of tocotrienols is the tocotrienol-rich fraction (TRF). This study aimed to investigate whether TRF induced liver Nrf2 nuclear translocation and influenced the expression of Nrf2-regulated genes. Methods: In the Nrf2 induction study, mice were divided into control, 2000 mg/kg TRF and diethyl maleate treated groups. After acute treatment, mice were sacrificed at specific time points. Liver nuclear extracts were prepared and Nrf2 nuclear translocation was detected through Western blotting. To determine the effect of increasing doses of TRF on the extent of liver nuclear Nrf2 translocation and its implication on the expression levels of several Nrf2-regulated genes, mice were divided into 5 groups (control, 200, 500 and 1000 mg/kg TRF, and butylated hydroxyanisole-treated groups). After 14 days, mice were sacrificed and liver RNA extracted for qPCR assay. Results: 2000 mg/kg TRF administration initiated Nrf2 nuclear translocation within 30 min, reached maximum level around 1 h and dropped to half-maximal levels by 24 h. Incremental doses of TRF resulted in dose-dependent increases in liver Nrf2 nuclear levels, along with concomitant dose-dependent increases in the expressions of Nrf2-regulated genes. Conclusion: TRF activated the liver Nrf2 pathway resulting in increased expression of Nrf2-regulated cytoprotective genes.
Ahmed Elbadri M Atia(9-2020)

Background: Libya has been malaria-free since 1973. The risk of malaria re-introduction to Libya is increasing because of the increase in imported malaria cases due to immigration to Libya from countries where malaria is endemic. Cases are mainly due to P. falciparum and Sub-Saharan Africa is the most common origin. This study was conducted to investigate the prevalence of malaria positive cases among illegal immigrants in the southern region of Libya. Methods: A prospective, observational, multi-center study was conducted. Three hundred and three illegal immigrants from 12 different countries were included. Enzyme-linked immunosorbent assay was used to detect the presence of serum malaria antibodies. Results: A total of 303 immigrants were included in the study with mean age of 25.78±5.92 years. Of them, 195 tested positive for malaria antibodies. Two hundred and sixty nine of the participants came from Brak Al-Shati, 16 from Sebha and 18 from Bergan centers, with 175, 15 and 5 positive cases, respectively. Most of the positive cases (172) arrived in Libya within 1-10 months. The highest number of positive cases (70) came from Ghana followed by (40) from Niger and (39) from Bangladesh. Conclusions: Illegal immigration is a major malaria re-introduction route. The issue of illegal immigration have to be treated urgently to stop the huge influx of illegal immigrants and increase the surveillance activities of infectious diseases in order to keep Libyan territories as a malaria-free lands. Key words: Illegal immigration, Malaria, Libya.
Fadwa Jamaledden Mustafa Kamel Mahanay, Badereddin Bashir K. Annajar, Asma A Ali Oun(11-2021)

Abstract Introduction: Provisional restoration is an important part of fixed prosthodontics. It must provide pulp protection, positional stability, occlusion maintenance , and space preservation. There are two methods for provisional restoration fabrication; direct and indirect techniques. Aim: the study aims to identify the most preferred technique of fabricating provisional fixed restorations according to different parameters and situations and to highlight the advantages and disadvantages of each technique, by specialist dentists practicing in different dental clinics in Tripoli. Methodology: In this study, 120 closed ended questionnaires were distributed to specialist dentists in dental fixed restoration among different private dental clinics in Tripoli/Libya to find out which technique they prefer to apply in their clinics. Results: This study has revealed that dentists prefer the direct technique for the anterior area and single unit and up to 3-4 units fixed partial denture. While indirect technique was more commonly used by dentists in cases of multi-units and posterior teeth. In addition, it has a higher success rate and does not cause pulpal and gingival irritation.
NARJES ELGHEZAWI HANAN IRHOMA , ABDUSSALAM ELJABALI (7-2021)