Background and Objectives: Compliance of community pharmacies with the proper practice of storage and dispensing of medicines is crucial for ensuring the quality and safety of medicines, particularly with the circumstance of recurrent electricity blackout in Libya. This study was aimed to assess the compliance of community pharmacies with the proper practice of drug storage in Tripoli city, Libya. Materials and Methods: A cross-sectional survey conducted in November 2020 targeting a total of 56 community pharmacies in Tripoli, Libya. The questionnaire was adapted from the WHO Checklist for Good Storage Practices and included 41 questions organized under five sections: socio-demographics, pharmacist’s attitude toward the quality of storage practices, queries on environmental storage conditions, the quality of facilities in the community pharmacy, and queries on storage and pharmacy practices. Data were presented as descriptive statistics. Results: Out of the total 56 visited pharmacies, a total of 46 (82.1%) pharmacists participated in the study with one pharmacist being interviewed in every pharmacy. Results showed that 15% of employees reported variety of cleanliness regulatory depending on cutting off running water circumstances in Tripoli and other reasons. About 78.8% participants observed dust in shelves and over the drugs packaging, and 22% of the participant pharmacies’ drugs exposure to direct sunlight. Additionally, 72% of employee experienced high temperatures in the pharmacy during electricity blackouts, whereas only 48% of them experienced humidity. In addition, 91.4% had alternative source of electricity, 44.3% had a power backup connected to the refrigerators. Conclusion: The compliance of majority of the community pharmacies operated in Tripoli is below standard. There is still need to improve the storage practices in the community pharmacies by obeying with the regulatory standards as specified by the Drug Regulatory Authority of Libya.
Ahmed Elbadri M Atia(5-2021)

The most common preparation of tocotrienols is the tocotrienol-rich fraction (TRF). This study aimed to investigate whether TRF induced liver Nrf2 nuclear translocation and influenced the expression of Nrf2-regulated genes. Methods: In the Nrf2 induction study, mice were divided into control, 2000 mg/kg TRF and diethyl maleate treated groups. After acute treatment, mice were sacrificed at specific time points. Liver nuclear extracts were prepared and Nrf2 nuclear translocation was detected through Western blotting. To determine the effect of increasing doses of TRF on the extent of liver nuclear Nrf2 translocation and its implication on the expression levels of several Nrf2-regulated genes, mice were divided into 5 groups (control, 200, 500 and 1000 mg/kg TRF, and butylated hydroxyanisole-treated groups). After 14 days, mice were sacrificed and liver RNA extracted for qPCR assay. Results: 2000 mg/kg TRF administration initiated Nrf2 nuclear translocation within 30 min, reached maximum level around 1 h and dropped to half-maximal levels by 24 h. Incremental doses of TRF resulted in dose-dependent increases in liver Nrf2 nuclear levels, along with concomitant dose-dependent increases in the expressions of Nrf2-regulated genes. Conclusion: TRF activated the liver Nrf2 pathway resulting in increased expression of Nrf2-regulated cytoprotective genes.
Ahmed Elbadri M Atia(9-2020)

Background: The concept of pharmacovigilance is not well known in Libya and its practice is still in the early stages. Aims: This study aimed to determine the knowledge, attitudes and practices of pharmacists in Tripoli, Libya about pharmacovigilance and the reporting of adverse drug reactions. Methods: A cross-sectional study was conducted from October 2019 to February 2020 of working pharmacists randomly selected from pharmacies in Tripoli. Participants were eligible for inclusion if they had a degree or diploma in pharmacy from a recognized university or institute. Data were collected using a validated self-administered questionnaire. Results: Of 500 pharmacists selected, 408 completed the questionnaire. The pharmacists' knowledge of pharmacovigilance and reporting of adverse drug reactions was poor overall: only 28.9% correctly defined pharmacovigilance and 14.7% knew about the existence of a centre for pharmacovigilance in Libya. The attitudes of the pharmacists to pharmacovigilance was positive: 77.2% believed that pharmacovigilance needed to be included in the pharmacy curriculum and 73.0% said that they would practice pharmacovigilance if trained. Pharmacists depended mostly on drug information leaflets to update their knowledge on adverse drug reactions. Conclusion: Given the pharmacists' low level of knowledge about pharmacovigilance but their readiness to become involved if trained, training programmes should be introduced for practising pharmacists to improve their knowledge and encourage their active participation in pharmacovigilance. Regulators need to reinforce the importance of reporting adverse drug reactions and implement pharmacovigilance policies in the Libyan health care system.
Ahmed Elbadri M Atia(7-2021)