The transcriptional changes that occurred in Salmonella enterica serovar Enteritidis during colonization of the alimentary tract of newly hatched chickens were studied. A whole genome oligonucleotide microarray was used to compare the expression pattern with that from bacteria cultured in nutrient broth in vitro. Amongst other changes Salmonella Pathogenicity Island (SPI)-1, SPI-2 and SPI-5 genes were up-regulated in vivo suggesting a close association with the mucosa during colonization. Particular attention was paid to genes associated with metabolism of dicarboxylic acids and to responses to high osmolarity. Association between the colonization phenotype and gene mutations indicated that the latter was more important as a contribution to the colonization phenotype. arabic 12 English 89
Abdulgader Dhawi, Elazomi A, Jones M A, Lovell M A, Li H, Emes R D, Barrow P A(7-2011)

Since its emergence, COVID-19 has greatly affected all aspects of life, and no country can be considered safe. Furthermore, it has resulted in great consequences, politically, economically, socially, and even ethically, which will be difficult to contain. Even highly developed countries have struggled to tackle this pandemic, and not all COVID-19 death cases were accurately reported. Strikingly, countries that reported the lowest numbers of cases at the early stage of the pandemic are the poorest and have the least resilient health systems, often ravished by civil war such as Libya, Sudan, Syria, and Yemen [1]. In Libya, the ability to react to the crisis is in doubt because of civil war and internal disarray. The health care system has been seriously affected, the country is divided, and two counterattack governments were appointed. The Ready Score parameter recommended by the WHO's Joint External Evaluation (JEE) applied to determine, stop, and prevent epidemics
Daw MA, Ahmed MO, ET AL.(1-2021)

Salmonella enterica can be considered as one of the most important causes of foodpoisoning with poultry thought to be the main source. Although S. Typhimurium, S. Enteritidis and the vast majority of other Salmonella serovars generally produce little systemic disease in adult chickens, they are able to colonize the alimentary tract of poultry. The two caeca are the main sites of the colonization of Salmonellae in chickens, and the bacteria can be easily harvested from the caeca for analysis. Bacterial proteins analysed utilizing SDS-PAGE showed differences between in vitro and in vivo that out of about 40 protein bands of in vitro preparation only a few (3-5) bands can be visualized from in vivo preparations. We suggested that some avian proteases might be responsible. Accordingly, and to investigate the hypothesis that bacterial-precipitated protein harvested from chickens is thought to be more protective than bacteria grown in broth culture, the immunogenicity of protein-precipitated vaccines harvested from chicken intestine and those from broth culture (in vitro), were compared using bacterial proteins as an orally inoculated vaccine candidate in chicken. The results demonstrated that the in vitro sonicated proteins obtained from a nutrient broth culture had a much better protective vaccine effect than the in vivo sonicated proteins preparations harvested from bacteria grown in chickens arabic 14 English 81
Altayeb Elazomi, Elhadi Araibi, Abdulgader Dhawi, Hatem Khpiza, Susan Liddell, Margret Lovell, Paul Barrow(12-2016)