Background: This study was conducted to investigate the presence of Bacillus cereus in meat, meat products, and some seafood in Libya. Materials and Methods: One hundred and thirty-one samples were collected from different geographic localities in Libya. The samples were subjected to microbiological analysis for enumeration and isolation of B. cereus by conventional cultural, biochemical, and molecular identification using polymerase chain reaction (PCR) and partial sequencing of 16S rDNA techniques. Results: Of 131 samples, only 38 (29%) isolates were found to be B. cereus based on their cultural characteristics on Mannitol Egg-Yolk Polymyxin (MYP) medium that included 30% beef, 38.2% beef products (minced, burger, kabab, and sausage), 31.8% camel meat, and 48% chicken products (burger, sausage, kabab, and liver). However, B. cereus was not detected from mutton and seafood samples. Seventeen isolates were subjected to molecular identification using PCR and partial sequencing of 16S rDNA technique and confirmed to be B. cereus. The confirmed B. cereus strains were tested for their antibiotic sensitivity profiles and showed a high percentage of multiresistance phenotype. Conclusions: The results provide a better understanding of B. cereus isolated from food of animal origin in Libya and suggest that meat and meat products might play an important role in the spreading of B. cereus through the food chain with antimicrobial resistance characteristics.
Ibrahim Eldaghayes(6-2018)

Infectious bronchitis virus (IBV) is a very dynamic and evolving virus, causing major economic losses to the global poultry industry. Recently, the Libyan poultry industry faced severe outbreak of respiratory distress associated with high mortality and dramatic drop in egg production. Tracheal and cloacal swabs were analyzed for several poultry viruses. IBV was detected using SYBR Green I real-time PCR detection based on the nucleocapsid (N) gene. Sequence analysis of the partial N gene indicated high similarity (~94%) to IBV strain 3382/06 that was isolated from Taiwan. Even though the IBV strain 3382/06 is more similar to that of the Mass type H120, the isolate has been implicated associated with intertypic recombinant of 3 putative parental IBV strains namely H120, Taiwan strain 1171/92 and China strain CK/CH/LDL/97I. Complete sequencing and antigenicity studies of the Libya IBV strains are currently underway to determine the evolution of the virus and its importance in vaccine induced immunity. In this paper we documented for the first time the presence of possibly variant IBV strain from Libya which required dramatic change in vaccination program.
Ibrahim Eldaghayes(12-2013)

Background: Pseudomonas aeruginosa is one of the most invasive organism that causes severe tissue damage in diabetic foot ulcers. A major problem in P. aeruginosa infection because of that it is commonly exhibits a high degree of resistance to antimicrobial agents .To improve appropriate antimicrobial therapy and reduce the incidence of antibiotics resistant bacteria, information on the antibiotic susceptibility to this bacterium is urgently needed. Therefore, the aim of this study was to isolate and determinate the antimicrobial susceptibility of the P. aeruginosa in diabetic foot ulcers patients. Methods: This study was carried out over the period between June 2014 to April 2015 at Tripoli Medical Center. A total of 120 bacterial isolates were cultured onto bacteriological media such as nutrient agar, MacConkey agar and blood agar. Identification of retrieved bacterial isolates was done using standard diagnostic microbiological laboratory methods and antibiogram was determined by VITEK ® 2 compact automated system. Results: Twenty one strains of P. aeruginosa from 120 diabetic foot ulcers were detected. P. aeruginosa isolates exhibited multidrug resistance to Ampicillin, Augmenting, Cefuroxime, Cefoxitin, Cefazolin, Ceftriaxone, Trimethoprim/sulfamethzole, Piperacillin. However, all isolates of P. aeruginosa were 100 % sensitive to Imipenem. Conclusion: P. aeruginosa infections of diabetic foot ulcers patients have multi-drug resistant. Imipenem is the empirical antibiotic of the choice. Key words: Pseudomonas aeruginosa, diabetic foot ulcer, antibiotics resistance arabic 17 English 114
Abdulkareem Elbaz, Abdulkareem Elbaz, Abdulgader Dhawi, Asma K. Elramalli, Ibrahim A. Algondi, , , Mustafa Saieh(12-2018)