The effect of low and high doses of Salmonella enteritidis PT4 (SE) on immunocompetent cells in caecum and spleen of one-day-old chicks was investigated. Subsets of T lymphocytes positive for CD3, CD4, CD8 and B lymphocytes (Bu1b-positive cells) were counted in the caecum after immunohistochemical staining and the relative percentage of these cells in the spleen was analysed using a FACScan cytometer on days 7, 10, 14, 21, and 27 post-inoculation (pi). In the low dose group, the number of CD3+ and CD4+ cells in the caecum had significantly increased at day 10 pi. Both CD8+ and Bu1b+ cells were significantly higher on day 14 pi in this group. In the high-dose group, the number of CD4+ cells had significantly increased at day 7 pi. CD3+, CD8+, and Bu1b+ cells showed prolonged proliferation at days 7 up to 21 pi. Splenic lymphocytes demonstrated significant changes only in the high dose group. The percentage of splenic CD4+ cells was decreased at day 7 pi. A decrease in CD3+ and CD8+ cells was found at day 14 pi in this group.
Abdulatif Asheg(11-2002)

A study was conducted to determine the effect of three native plants from El-Jabal al ELAkhdar, (Libya) on performance and cecal coliform count of broiler chickens. A total of 1260 one-day-old male Cobb chickens were used in the experiment. The birds were assigned to 7 treatment groups (6 replicates per treatment). The dietary treatments included basal diet with no additive (control), and 6 other dietary treatments (Arbutus pavarii, Salvia officinalis and Zizyphus Vulgaris) each of which was added at the rate of 0.5 g and 1 g/kg of basal diet. Results explicitly revealed that all dietary treatments had a significant effect on body performance of broiler chickens compared to the control with the exception of the dietary treatment of S. officinalis at dosage of 0.5 g/kg that has expressed noticeable reduction in body weight. Coliform counts in the cecum of birds receiving 1% A. pavarii and 1% Z. Vulgaris were significantly lower (P ⩽ 0.05) than those of control group from early weeks of treatments, whereas all plant shows a significant lowering (P ⩽ 0.05) of cecal coliform count during the rest of experiment compared to control group. These results emphasize the potential biotic role of such plants together with the immune modulating effects on treated birds. However, further pharmacological and clinical work should be adopted in the future to present an obvious understandable theory behind the potential beneficial as well as side effects of such natural plants.
A. A. Asheg (12-2015)

Chicks (1-d-old, three groups, each containing 50 chicks) were inoculated with 2×102 and 2×108 CFU ofSalmonella enteritidis; the third group were kept as uninoculated control. Five birds from each group were euthanized at intervals from 6 h to 4 weeks post-inoculation (pi). In the lowdose groupS. enteritidis was isolated from 60% cecal samples at 18 h pi. and from 20% of livers at 3 d pi. Individual variation in the frequency ofS. enteritidis recovery was observed in this group. The clearance of salmonella from the organs was faster in the low-dose group, and salmonella was not isolated from the liver and cecum at 21 and at 27 d, pi, respectively. However, in the high-dose group,S. enteritidis was isolated from all ceca and 80% of liver 6 h pi, and salmonella was detected in the cecum and liver throughout the experiment. Serous typhlitis and unabsorbed yolk sac were the most prevalent lesions in both groups. Granulomatous nodules in the cecum were found occasionally in some cases in both inoculated groups, which can play a role as reservoirs in carrier chicks.
A. A. Asheg(10-2001)

Adhesion and colonization of high (2 × 108 CFU) and low doses (2 × 102 CFU) ofSalmonella enteritidis (phage type 4) was determined in the ceca collected 6 h-4 weeks after inoculation (pi), of 1-d-old White Plymouth Rock orally-inoculated chickens.S. enteritidis was associated with the epithelial surface of the villi in the low-dose group 18 h-7 d pi, the penetration in the cecal lamina propria was observed on day 1 and 10 pi. In the high-dose group, adhesion and colonization was observed in all birds killed 6 h-14 d pi; penetration of the bacteria into the cecal lamina propria was seen 1–21 d pi. Large numbers of macrophage-like cells containingS. enteritidis were observed in the cecal lamina propria on days 3–21 pi. Colonization and migration byS. enteritidis in the intestinal tract of chickens was shown to be dose dependent.
A. A. Asheg(3-2003)

To determine the effect of different doses of Salmonella enteritidis on immunocompetent cells, the thymus, bursa of Fabricius, and peripheral blood were examined. One-day-old chickens were orally infected with 2 × 102CFU/ml (low dose) and 2 × 108 CFU/ml(high dose) of S. enteritidis PT4. Subsets of T lymphocytes (CD3, CD4, and CD8), and BU1b cells using an indirect immunofluorescent method and flow cytometry were analysed on days 7, 10, 14, 21, and 27 postinoculation (dpi). The actual number of lymphocytes in the peripheral blood showed a significant increase in the low dose group (P < 0.05) on day 21 pi, and in the high dose group on day 27 pi (P< 0.001) compared to controls. The increase of CD3+, CD4+, and CD8+ T cells was observed in both infected groups compared to the controls from day 14 pi, significant on day 21 pi (P < 0.05) in the high dose group. The subpopulation of CD8+ was higher also on day 27 pi (P < 0.01) compared with the values of the control group. The subpopulation of BU1b+ B cells in both infected groups showed higher values, but without significant differences from controls. The thymus T lymphocytes showed a significant decline in CD3+ T cells on day 7 pi, whereas CD4+ T cells were significantly (P < 0.05) increased on day 7 pi in both infected groups. The bursal lymphocytes showed a decrease in BU1b+ B cells in both infected groups compared with the control group, significant in the high dose group on day 21 pi (P < 0.05). These results indicate that S. enteritidis infection induced the changes in immunocompetent cells, included cellular and humoral immune response. Salmonella infection accelerated the maturation and differentiation of thymus T cells in the first phase of infection, what was secondarily reflected by increased number of studied T lymphocyte subpopulations in the peripheral blood from day 14 to 27 after infection. Similarly, it appears that the activation of B cells in bursa of Fabricius caused the decrease number of bursal B lymphocytes and increased number of these cells in the peripheral blood from day 14 to 27 pi in both infected groups.
A. A. Asheg(3-2003)

A study was conducted to determine the effect of three native plants from El-Jabal al ELAkhdar, (Libya) on hematological profile, cholesterol and immune response to ND vaccine in broiler chickens. A total of 1260 one-day-old male Cobb chickens were used in the experiment. Chickens were assigned to 7 treatment groups (6 replicates per treatment). The dietary treatments included basal diet with no additives (control), and 6 other dietary treatments (Arbutus pavarii, Salvia officinalis and Zizyphus Vulgaris) each of which was added at the rate of 0.5g and 1g/kg of basal diet. Results explicitly revealed that no significant change in TLC between the treated and control groups has been recorded. A significant heteropenia was recorded at the third week of treatment with 1% A. Pavarii 0.5 S. Officinalis and 0.5 Z. Vulgaris when compared to control group. However, the lymphocytic count show a significant increase (P≤0.05) in all treated groups compared to the control groups. Immunologically, an enhanced humeral immune response was very obvious based upon the significant elevation of antibody titer at the third week of treatment for all groups received 1 % of the three types of plants. Interestingly , cholesterol levels were significantly elevated at the six week of treatment with 0.5 % Z. Vulgaris while highest decline in cholesterol levels were recorded in group that have received 0.5 S. Officinalis.
ِAbdulatif A. Asheg(3-2015)

In order to study microscopic and macroscopic lesions in chickens infected with low and high doses of Salmonella enteritidis PT 4 (SE), 150 one-day-old White Plymouth Rock chicks were divided into three groups each containing 50 birds. The chickens of the first and second groups were inoculated with 2 x 102 and 2 x 108 cfu of Salmonella, respectively. The chicks of the third group were kept as uninoculated control birds. Five birds from each group were euthanized by cervical dislocation at 6, 12, 18 hours post inoculation (hpi) and then after 1, 3, 7, 10, 14, 21, and 27 days post inoculation (dpi) and were examined for presence of gross and histological lesions. Catarrhal typhlitis and unabsorbed yolk sac were the most prevalent gross lesions in both groups. Histologically, granulomatous nodules in the caecum were found occasionally in some cases in both inoculated groups. The present study demonstrated that the chickens infected with a low dose of SE showed fewer lesions, which were milder in comparison with the birds inoculated with a high dose of SE. It suggests that chickens were able to capture the pathogen in the low dose group
Abdulatif Asheg(1-2001)

This study was conducted to investigate the prevalence of Newcastle Disease, Avian Influenza, Infectious Bronchitis, and Infectious laryngotracheitis in some regions of Tripoli-Libya. Serum samples were collected from Four broiler farms in Wadi Alrabea area, two layer farms and one backyard farm in Gaser Bin Gasher, two layer farms in Alswani and one backyard farm in Zliten City and screened for the presence of antibodies against LPAI-H9, HPIA-H5, HPIA-H7, ND, IB 4/91 strain using the HI test and antibodies against ILT virus by ELISA. The overall seroprevalence of H9, H5, H7, ND, IB4/91 and ILT was 26.5%,0%,0%,, 29.5%,43% and 30% respectively. All sera tested for AI-H5 and AI-H7 were negative. In Wadi Alrabea flocks, seroprevalence of H9, H5, H7, ND, IB4/91 and ILT was 44%, 0%, 0%, 16.28%, 32.56% and 0% respectively. Where as in Gaser Bin Ghasher, was 24%,0%, 0%, 33.87, 36.29% and 39% respectively. In Alswani, the seroprevalence was 8.70%, 0%, 0%, 52.17%, 54.35% and 54.3%. The seroprevalence of H9, H5, H7 in Gaser Bin Ghasher backyard chickens was 23%, 0%, 0% respectively and the seroprevalence of H9, H5, H7, ND, IB4/91 of Zliten backyards was 12%, 0%, 0%, 12%, 96% respectively. Further investigations are recommended to identify the circulating virus genotypes and models of transmission for better understanding of these viruses epidemiology in backyard chickens in Libya. Vaccination of backyard chickens against ND using heat resistant strain is highly recommended.
Housen Ashareef Mohammed, Abdulatif Abdulaziz Asheg (10-2021)

This study amid to determine the prevalence of Salmonella enteritidis and multi-drug resistant in some chicken slaughterhouses in Tripoli -Libya. The study include three regions of Tripoli namely south, East and West regions. Five slaughterhouses were selected from each region. every chicken slaughterhouse was visited 3 time for sample collection with 2 weeks interval. Samples were collected from neck skin, crop and spleen from 5 chicken. Total of samples from all regions were 675. Every 5 samples were processed for isolation and identification by biochemical identification and serotyping and antibiotic sensitivity test. The total rate of isolation of Salmonella spp was found to be 15% and Salmonella enteritidis was 7%. The highest prevalence 9% of Salmonella enteritidis was recorded in south region of Tripoli, however, the highest prevalence of Salmonella spp was found in West region. the prevalence of Salmonella was significantly (P
Abdulatif Asheg, ’ohamed Fayz Othman(1-2019)

ِAbdulatif Asheg, Khalid Fathi Almasri (1-2020)

LOCUS KF632582 547 bp DNA linear VRL 07-DEC-2013 DEFINITION Gallid herpesvirus 1 strain ILT-1 glycoprotein G gene, partial cds. ACCESSION KF632582 VERSION KF632582.1
Abdulatif Asheg(9-2013)

Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae Streptococcus.
Abdulatif Asheg, Eldin Eissa,A(9-2021)

Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus.
Abdulatif Asheg, Eldin Eissa,A.(9-2021)

Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Avulavirinae; Orthoavulavirus.
Abdulatif Asheg, Abdulwahab Kammon(5-2015)

Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus.
Abdulatif Asheg, Abdulwahab Kammon(5-2015)

Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio.
Abdualtif Asheg, Eissa,A., Abdelsalam,M(6-2014)

Influenza A virus (A/chicken/Libya/13VIR7225-5/2013(H9N2)) segment 4 hemagglutinin (HA) gene, partial cds.
Kammon,A, abdulatif Asheg(9-2015)

Larynotrachitis (LT) is a viral respiratory tract infection of chickens that may result in severe production losses due to mortality and decrease egg production. Samples (live and dead birds) from two flocks of 30 weeks old layers (Iza Brown) were admitted to our department. The clinical signs, gross and microscopic
Abdulatif Asheg (9-2007)

Detection and molecular characterization of infectious laryngeotracheitis in laying hen in Libya
Abdulatif Asheg(9-2013)

Effect of organic acids on cecal bacteria count in chicken under heat stress
Abdulatif Asheg(1-2013)

Investigation on the presence of antibodies against Chicken Infectious Anemia in Libya using the Enzyme-Linked Immunosorbent Assay
Abdulatif Asheg(4-2005)