ABSTRACT Biocides (disinfectants and antiseptics) are used extensively in hospitals and other health care settings for a variety of applications. However, their antibacterial effectiveness is not always well stated by the manufacturers and consumers find it difficult to choose the right product according to their needs. Therefore, the aim of this study was to evaluate practically achieves disinfection efficacy of locally available disinfectants and antiseptics on surfaces and infectious microbiological utilities of Tripoli Medical Center (TMC). Fourteen biocides; Ten disinfectants (Dex36, Dex50, Dex53, Mzid-S, Mzid-AF, Deson, Dettol, Seem, Chlor and Wipol) and four antiseptics (HiBi, Esept, Emed and Desman) were tested at concentrations recommended by manufacturers on surfaces, walls and different utilities that were contaminated by locally circulating six isolates of Bacillus cereus, Staphylococcus haemolyticus, staphylococcus aureus, Klebscilla pneumonia, Acinetobacter baumannii, and Pseudomonas aeruginosa using four standard evaluation test procedures (capacity test, diffusion test, in-use test and suspension test). Results showed the highest average log reduction (7.4) of test bacteria was given by quaternary ammonium based disinfectants; Dex50, Dex53, Deson-AF and Mzid-S. A comparable average log reduction of test bacteria was noted (7.0, 7.2 and 7.3) for Deson-AF, Dex.50 and Dex.53 respectively. In contrast HiBi as an alcoholic based antiseptic gave the highest average log reduction (7.2) against tested bacteria. Desman, Emed and Wipol antiseptics had poor antibacterial activity on tested bacteria causing almost undetectable log reduction in cell viability. From contamination point of view, five critical departments in Tripoli Medical Centre (TMC) namely; Neonatal Intensive Care Unit (ICU), Surgical ICU, Dermatology, Oncology, and Urology departments were found contaminated with six types of bacteria. Bacillus cereuses followed by Klebscilla pneumonia were the most bacteria spread in whole five mentioned departments, particularly in dermatology department. Staphylococcus haemolyticus was next most widespread bacterium and almost isolated equally from the five medical departments. Acinetobacter baumannii had been isolated from Neonatal ICU; whereas Staphylococcus aureus and Pseudomonas aeruginosa had been isolated from Oncology Department and Surgical ICU respectively. In addition, Dex36, Chlor, Chlor, Wipol, Desman and E-med exhibited bacterial contamination in both capacity and in-use tests. In conclusions, quaternary ammonium compounds (QACs) and combination of QACs with aldehyde formulations were found to be the best disinfectants for disinfection of contaminated surfaces.
آمـــال منصــــور ابراهيـــم(1-2015)

المقدمة الحمدددلله ب ال المددد لملصلا اللددد ع اليددد نبدددا يدددلللهلم منللمددد يدددلللله الم يدددبلص لبا الله نبله يب - نبا آله لحنه –محملله نمدددلله لا ف ل ل تمددد مدددللهل مدددص ددد ل المظ لددد ل الممم ل لددد نقدددا اع الددد ل الأللهندددولا مدددص م اً ا تأثل المممل ً كث امتللهالله فو ي ط المقل لله المثقفلص ل ل ص نلله اي ذا الم . ال ل تمددد لت الت ددد ل ت لتيددد نددد لث اا التمددد م ضددد لا ل لددده تدددأثل نددد فدددو تطددد اللله اي ل الأللهنل لا تأثل خ ل فو الخط ا المقللهي الأللهنولا مع نلله إغفد مد لتمتدع نه مص مل تأ لخل فو التا ث الم نولا نمو جذ ه المتمثب فو (اليد ا ل ال دم ل لا التضملص الااتن س ) ً مقللهل ً للس ملطبح فقطلا م لمثلبده مدص مضدم ص م دت ت ال مع تا ث ال م ا ن م . للها ي ال ل تم ل ايدتق له الموند ج ال لللهحض يط ع امغ الدم لا لده المتدده المظ لدد فم للتدده اةجا لدد فددو نددط الم ضددو ن لح ضدد مددص خدد ايتحضدد الملل الأللهنل القللهلم نطه نل ع م ل . الله هلله مفهد ال ل تمد دً خبط تدللهاخ ً نلمده ندلص المفد ل الأخد ك ك ليد ا ل ال م ل لا التضدملصلا الااتند سلا متلجد الااتدا ا ندلص دذا المفهد تبد المفد ل فلمد ج ه الم نلمه فو الت ال التأثل ِّ ل ل الات لخ . لأ مل م ذك م مص الحدللهلث نمده ال اختد ل لله ايدتو ندص ل تمد تطنلقده نبدا للهلددد اص ال ددد ن اندددص تدددَ ن دددد)لا كددد ص يدددنا اختلددد ي لهدددذا الدددللهل اص 867 م الملددد ي (ل لضخ مته كث ع الملل التدو ل تمد فلهد مدع ملد اللهلمد لا يد اا كد ص ال ل تمد ً من دد ا ً غلدد من دد الا فضدد ً نددص دده ع انددص تددَ ن م مددللهل دد ال ددم اا الملدد ي الددذي ل المل للص فو المل الممب كولا ال ل م ه ت إذ ن لله ً ً إلج نل مع التا ث الم ندو الدذي -‌2‌- ثقفددهلا تجبلددل نمدد للهه المملقدد فددو ددم ه الددذي لفدده نأيددب ا فمددو متمللددو جيلددلله ثدد ددذا التا ث الم نو فو خل له.
حمزة شعبان الأحول(1-2015)

Abstract An increased emphasis has been placed on the microbiological analysis of raw milk and dairy products designed to evaluate quality and to ensure safety for human consumption. Many microorganisms such as Escherichia coli can contaminate raw milk during milking process or via environmental contamination during different milk-processing methods. Enterohaem- orrhagic Escherichia coli O157 (EHEC) are a major cause of food-borne illnesses in humans. A hundred and seven samples of milk and milk products were collected from some regions (Janzour, Tripoli, Kryamia, Tajoura and Toubrok) in Libya. In the current study, samples were subjected to microbiological analysis for enumeration and isolation of Escherichia coli, which were detected by conventional cultural and molecular method using polymerase chain reaction (PCR) and partial sequencing of 16S rDNA. Out of the107 samples; 26 positive sample according to culture characteristics were found to be EHEC O157 included cow's milk 10 % (3/ 28), she-camel's milk 33%(3/ 9), goats milk 17 % (1/6) and 25 % (7/28) fermented raw milk samples. Samples of fresh locally made soft cheese (Maasora and Ricotta) were 43% (9/21) and 30% (3/10) respectively, while none of the ice cream samples revealed any growth, but 12 of these 26 sample were confirmed by partial sequencing of 16SrDNA as Escherichia coli (E. coli). Key words: Enterohaemorrhagic Escherichia coli; raw milk; 16S rDNA.
إيناس ماهر عوض علي(1-2016)