Aims: Diabetic foot ulcer is a significant complication of diabetes mellitus and often proceed lower extremely amputation. Propolis is a naturally occurring anti-inflammatory bee derived protectant resin. Previously, topically applied propolis has been reported to reduce inflammation and improves cutaneous ulcer healing in diabetic rodents. This study aimed to determine the Libyan honey and propolis activity and honey against bacteria isolated from diabetic foot ulcer lesion Study Design: In vitro antimicrobial activities of honey and crude hexane and methanolic extract of Libyan propolis against bacteria isolated from diabetic foot ulcer lesion Place and Duration of Study: Samples collected from patient in Tripoli Iben Nafees Hospital using disc and agar diffusion method. Methodology: Disk diffusion method on groups of aerobic and anaerobic bacteria were obtained from diabetic foot lesion. Results: The result showed that the percentage of aerobic bacteria isolated from diabetic lesion was about 74%, which include MRSA, E. coli, Ps. aeruginose, Citrobacter, Pantoea, Proteus, Staphylococcus epidermis, Enterobacter and Serratia bacteria. Whereas anaerobic bacteria reported about 26% of Clostridium, Bacteroides, and Lactobacillus Jensenii. Conclusion: It was concluded that the honey and propolis extract had antibacterial activity against a different type of aerobic and anaerobic bacteria that were isolated from diabetic foot ulcer lesion.
Basma Mohamed K Doro(1-2019)

virus infection continues to be an important public health concern with regard to blood transfusion in Libya and in Africa. This concern is related to the screening test. Objectives: The main aim of this study to investigate the blood donors samples for HIV, HBV and HCV infections in Tripoli-Libya, North Africa during the first five months of 2015. Methods: The total of 686 blood samples obtained from healthy blood donors who attended Tripoli’s central blood bank, were tested for HBsAg, HCV and HIV using the VITROS® 3600 Immunodiagnostic System. Results: From the 686 samples examined, the frequency of HBsAg positive cases was 0.8%, the number of anti-HBc positive samples was found to be particularly high in the age group 29 and 36 years (p= 0.0001). The number of anti-HBc positive samples was found to be particularly high in the age group 30-39 years (p= 0.01). Most occupation that had positivity with anti-HBc and HBV-DNA were free workers and was less in students. Most positive cases were from east of Tripoli the capital (Tagora, Soq-Aljomaha). Conclusion: The frequency of HBsAg positive blood donors and anti-HBc among this sample was 0.8% and 0.7% respectively, which is low compared with the international findings. The current study estimated the expected exclusion rate of anti-HBc and HBsAg positive donated blood, as this would be an important factor to consider before donation. arabic 16 English 63
Basma Mohamed K Doro(9-2015)

More than 2000 cultivars of Phoenix dactylifera L. are known to grow around the world. Date is an essential fruit in North Africa, and in Libya in particular, it represents an important national food resource. Recently, researchers discovered the medicinal value of different parts of palm tree beside the well known nutritional value of the fruit edible part. In this study five Libyan date seeds (Bestian, Deglet, Abel, Khadrai and Hamrai) were collected from Aljofra region and were screened for phytochemical components and their antimicrobial activity. The phytochemical analysis of methanolic extract of five different Libyan date palm seeds showed the presence of aflavnoids, glycosides, phytosterols, phenolic compound, tannins, and fixed oil, absences of alkaloids and saponine. The susceptibility of bacterial species to five methanolic extracts of Phoenix dactylifera L seeds by cup-cut agar method exhibited Inhibition zones (IZD). That inhibition zone diameters are vary from 15.0 0.3 to 3.02 0.01 against MRSA; 16.040.1 to 6.0  0.7 against Pseudomonas aeruginosa; 14.2 0.1 to 6.00.7 against Proteus vulgaris; 18.3 0.2 to 6.0 0.1 against Staphylococcus aureus; 14.04 0.2 to 2.0 0.02 against E. coli; and 18.0 0.1 to3.2 0.02 against Klebsiella pneumonia. The results of current study shows the antibacterial properties of five Libyan samples of date seed extract against a wide spectrum of bacteria. arabic 21 English 126
Basma Doro, Nahla Labyad, Fadia Gafri(1-2020)