Aims: Diabetic foot ulcer is a significant complication of diabetes mellitus and often proceed lower extremely amputation. Propolis is a naturally occurring anti-inflammatory bee derived protectant resin. Previously, topically applied propolis has been reported to reduce inflammation and improves cutaneous ulcer healing in diabetic rodents. This study aimed to determine the Libyan honey and propolis activity and honey against bacteria isolated from diabetic foot ulcer lesion Study Design: In vitro antimicrobial activities of honey and crude hexane and methanolic extract of Libyan propolis against bacteria isolated from diabetic foot ulcer lesion Place and Duration of Study: Samples collected from patient in Tripoli Iben Nafees Hospital using disc and agar diffusion method. Methodology: Disk diffusion method on groups of aerobic and anaerobic bacteria were obtained from diabetic foot lesion. Results: The result showed that the percentage of aerobic bacteria isolated from diabetic lesion was about 74%, which include MRSA, E. coli, Ps. aeruginose, Citrobacter, Pantoea, Proteus, Staphylococcus epidermis, Enterobacter and Serratia bacteria. Whereas anaerobic bacteria reported about 26% of Clostridium, Bacteroides, and Lactobacillus Jensenii. Conclusion: It was concluded that the honey and propolis extract had antibacterial activity against a different type of aerobic and anaerobic bacteria that were isolated from diabetic foot ulcer lesion.
Basma Mohamed K Doro(1-2019)

Toxoplasmosis is a zoonotic disease caused by Toxoplasma gondii. Risk factors include consumption of undercooked meat, raw vegetables, and unfiltered water. Aim: This study aims to screen antibodies titer in toxoplasmosis positive patients in Asabieh city in Libya and to screen risk factors associated with infection in this area. Study design: Data were collected using a cross-sectional design. Study Duration and Location: Data were collected from patients of different ages and gendersattending Ali Omar Ascar hospital, Asabieh, during the period of January 2017 to January 2018. Methodology: A single blood sample was collected from 150 patients and spun at 3000 rpm to obtain serum. The serum samples were analysedto detect anti-Toxoplasma IgG and IgM antibodies using enzyme linked immunosorbant assay (ELISA, BioChec) kit according to manufacturer’s instructions. A self-structured questionnaire was used to obtain information on farm animal’s contact, the process of vegetables washing, meatcooking, Water resource and raw water consumption. Results: The total seroprevalence of toxoplasmosis was 78.6%,out of which IgG and IgM were 68%, and 77.4% respectively. Several risk factors were identified, including daily contact with farm animals (82.6% were +ve, P = .0.01), unfiltered water (61.3% were +ve; P = 0.003). Conclusion: asabieh area showed a high prevalence of toxoplasmosis, and many environmental risk factors associated with the infection as animal hosts, human lifestyle were also identified, that could help to reduce the risk of spreading and transmission of infection among the populations in the future.
Basma Doro, Hend Shubar, Asma Ramadan, Badereddin Annajar(8-2019)

Leishmaniasis is a major health problem and itis estimated that 12 million people are currently infected.A vaccine which could cross-protect people against different Leishmania spp. would facilitate control ofthis disease as more than one species of Leishmania may be present. In this study the ability of a DNA vaccine, using the full gene sequence for L. donovani gamma glutamyl cysteine synthetase (GCS) incorporated in the pVAX vector (pVAXGCS), and a protein vaccine, using the corresponding recombinant L. donovani GCS protein (LdGCS),to protect against L. major or L. mexicana infection was evaluated. DNAvaccination gave transient protection against L. major and no protection against L. mexicana despite significantly enhancing specific antibody titres in vaccinated infected mice compared to infected controls. Vaccination with the LdGCS protected against both species but only if the protein was incorporated into non-ionic surfactant vesicles for L. mexicana. The results of this study indicate that a L. donovani GCS vaccine could be used to vaccinate against more than one Leishmania species but only if the recombinant protein is used. arabic 30 English 176
Basma Doro, (12-2012)