The present study investigated the effects of an aqueous extract of green tea (Camellia sinensis) on kidney functions through Urea, uric acid and creatinine measurement within male Wistar albino rats intoxicated with nicotine. Forty male Wistar albino rats were randomized into four groups of ten. Over a 28 day period the control group received daily subcutaneous dose of saline (10 ml) and drank only water, the green tea group received subcutaneous dose of saline (10 ml) and drank only green tea, the nicotine group received subcutaneous dose of nicotine (10 ml saline with 3mg/kg weight of nicotine) and drank only water, and the green tea with nicotine received subcutaneous dose of nicotine (10ml saline with 3mg/kg weight of nicotine) and drank only green tea. Urea, uric acid and creatinine were measured by ultraviolet spectrophotometer utilizing standard methods. No differences were observed between the control and green tea only groups, and no differences were found for creatinine levels between any of the groups. The Nicotine group had urea and uric acid concentrations significantly (P < 0.05) greater than those of the control, green tea only groups, and the green tea with nicotine groups. The results of the present study indicate that an aqueous extract of green tea reduced the effects of nicotine on urea and uric acid concentrations. arabic 10 English 59
Marwan Mustafa Ali Draid(6-2016)

Efficient extraction of genomic DNA (gDNA) from biological materials found in harsh environments is the first step for successful forensic DNA profiling. This study aimed to evaluate two methods for DNA recovery from animal tissues (livers, muscles), focusing on the best storage temperature for DNA yield in term of quality, quantity, and integrity for use in several downstream molecular techniques. Six male Swiss albino mice were sacrificed, liver and muscle tissues (n=32) were then harvested and stored for one week in different temperatures, -20C, 4C, 25C and 40C. The conditioned animal tissues were used for DNA extraction by Chelex-100 method or NucleoSpin Blood and Tissue kit. The extracted gDNA was visualized on 1.5% agarose gel electrophoresis to determine the quality of gDNA and analysed spectrophotometrically to determine the DNA concentration and the purity. Both methods, Chelex-100 and NucleoSpin Blood and Tissue kit found to be appropriate for yielding high quantity of gDNA, with the Chelex100 method yielding a greater quantity (P < 0.045) than the kit. At -20C, 4C, and 25C temperatures, the concentration of DNA yield was numerically lower than at 40C. The NucleoSpin Blood and Tissue kit produced a higher (P=0.031) purity product than the Chelex-100 method, particularly for muscle tissues. The Chelex-100 method is cheap, fast, effective, and is a crucial tool for yielding DNA from animal tissues (livers, muscles) exposed to harsh environment with little limitations.
Huda H. Al-Griw, Zena A. Zraba, Salsabiel K. Al-Muntaser, Marwan M. Draid, Aisha M. Zaidi, Refaat M. Tabagh , Mohamed A. Al-Griw(8-2017)

The present study aimed at evaluation of the immuomodulatory effect of sulphadimidine by investigating its ability to affect constitutive expression of MHC-II molecules on B-cells, which are one determinant of antigen presentation and the vigor of immunity. Three groups of broiler chicken were used, the first two groups received 100 mg/kg and 200 mg/kg sulfadimidine, respectively, in drinking water; while the third group was left as control. Bursal samples were taken after 7 and 14 days of treatment and checked for MHC-II gene expression by RT-PCR technique. The obtained results demonstrate that oral administration of sulphadimidine inhibited the expression of MHC-II mRNA in bursal of Fabricius of broiler chicken in a dose and age dependent manner. This finding is of clinical importance as many farmers routinely add sulpha drugs to drinking water as a prophylactic measure against some infections; yet the data presented in this study doesn't recommend this managing behavior. arabic 11 English 83
Marwan Draid(5-2012)