Efficient extraction of genomic DNA (gDNA) from biological materials found in harsh environments is the first step for successful forensic DNA profiling. This study aimed to evaluate two methods for DNA recovery from animal tissues (livers, muscles), focusing on the best storage temperature for DNA yield in term of quality, quantity, and integrity for use in several downstream molecular techniques. Six male Swiss albino mice were sacrificed, liver and muscle tissues (n=32) were then harvested and stored for one week in different temperatures, -20C, 4C, 25C and 40C. The conditioned animal tissues were used for DNA extraction by Chelex-100 method or NucleoSpin Blood and Tissue kit. The extracted gDNA was visualized on 1.5% agarose gel electrophoresis to determine the quality of gDNA and analysed spectrophotometrically to determine the DNA concentration and the purity. Both methods, Chelex-100 and NucleoSpin Blood and Tissue kit found to be appropriate for yielding high quantity of gDNA, with the Chelex100 method yielding a greater quantity (P < 0.045) than the kit. At -20C, 4C, and 25C temperatures, the concentration of DNA yield was numerically lower than at 40C. The NucleoSpin Blood and Tissue kit produced a higher (P=0.031) purity product than the Chelex-100 method, particularly for muscle tissues. The Chelex-100 method is cheap, fast, effective, and is a crucial tool for yielding DNA from animal tissues (livers, muscles) exposed to harsh environment with little limitations.
Huda H. Al-Griw, Zena A. Zraba, Salsabiel K. Al-Muntaser, Marwan M. Draid, Aisha M. Zaidi, Refaat M. Tabagh , Mohamed A. Al-Griw(8-2017)

Background: The present work was conducted to investigate the effects of lead acetate intoxication on glucose and liver functions in albino rats, and the possible effectiveness of using camel milk to protect against lead induced toxicity. Methods: Eighteen male albino rats were divided into three groups of six, the first was a control group, the second received orally lead acetate in water as (2 ml saline containing 5 mg/kg body weight of lead acetate) and the third received the same lead acetate dose and supplemented with 2 ml of camel milk, the experiment lasted for three weeks. Results: The results indicated that exposure of animals to lead acetate caused a significant increase (p
Marwa M. Draid(6-2016)

Several molecular techniques that are commercially described in the literature for early detection of blood stream infection in attempting to overcome the limitations of the gold standard blood culture. SepsiTest™ is aCE marked commercial platform that has been described in the literature. However, there were no studies addressing the accuracy of the test as a diagnostic platform for detection bacteraemia in whole blood samples. The study, conducted to investigate and discuss the strategy of SepsiTest™ in comparisonto our previously validated real-time PCR based assays (BactScreen™) and the previously published “in-house” minor groove binder (MGB)-based all bacteria assay. The three assays showed different sensitivity patterns for detecting bacteria in blood stream pathogens in favouring of using BactScreenTMtest. SepsiTest™ could be valuable but their lowest sensitivity in addition to their use of the unspecific SYBR Green fluoresce dye that question its diagnostic accuracy could be used as a last choice molecular diagnostic technique for detection of bacteraemia in whole blood samples. However, SepsiTest™ strategy rather, may provide useful diagnostic tool for detecting live pathogens in food technology arabic 32 English 130
Marwan Draid(12-2016)