Efficient extraction of genomic DNA (gDNA) from biological materials found in harsh environments is the first step for successful forensic DNA profiling. This study aimed to evaluate two methods for DNA recovery from animal tissues (livers, muscles), focusing on the best storage temperature for DNA yield in term of quality, quantity, and integrity for use in several downstream molecular techniques. Six male Swiss albino mice were sacrificed, liver and muscle tissues (n=32) were then harvested and stored for one week in different temperatures, -20C, 4C, 25C and 40C. The conditioned animal tissues were used for DNA extraction by Chelex-100 method or NucleoSpin Blood and Tissue kit. The extracted gDNA was visualized on 1.5% agarose gel electrophoresis to determine the quality of gDNA and analysed spectrophotometrically to determine the DNA concentration and the purity. Both methods, Chelex-100 and NucleoSpin Blood and Tissue kit found to be appropriate for yielding high quantity of gDNA, with the Chelex100 method yielding a greater quantity (P < 0.045) than the kit. At -20C, 4C, and 25C temperatures, the concentration of DNA yield was numerically lower than at 40C. The NucleoSpin Blood and Tissue kit produced a higher (P=0.031) purity product than the Chelex-100 method, particularly for muscle tissues. The Chelex-100 method is cheap, fast, effective, and is a crucial tool for yielding DNA from animal tissues (livers, muscles) exposed to harsh environment with little limitations.
Huda H. Al-Griw, Zena A. Zraba, Salsabiel K. Al-Muntaser, Marwan M. Draid, Aisha M. Zaidi, Refaat M. Tabagh , Mohamed A. Al-Griw(8-2017)

The relationship between ATP-induced membrane potential and contraction was inestigated in the smooth muscle of chicken anterior mesenteric artery using micro-electrode and tension recording techniques respectively. Application of ATP to en-dothelium-denuded arterial preparations developed concentration-dependent, slow depolarization which started only at concentrations not less than 10 μM. On the other hand the tension recording experiment revealed that ATP produced concentration dependent contractions, which were evident at concentrations lower than those evoked depolarizing response to ATP (as lower 100 nM). Both depolarizing & contracting responses were abolished by the P2X receptor blocker, PPADS (50 μM) but not by P2Y receptor blocker CBF3GA (100 μM), indicating that the excitatory responses of ATP were mediated via activation of P2X receptor. These findings suggest that ATP-mediated contraction is not dependent on membrane depolarization only, at least at low concentrations in the first order branches of chicken anterior mesenteric artery. arabic 21 English 153
Marwan Draid(7-2012)

Nerves and Endothelial Cells in the Chicken Anterior Mesenteric Artery arabic 16 English 118
Marwan Draid(1-2006)