Abstract This study is to identify some mutations in exons 23, 24, 26 A+B, and 26 A of the factor VIII gene that causes hemophilia A among Libyan patients. Forty-three patients (40 males, 3 females), 10 carrier, and 10 control (8 males, 2 females) were enrolled in this study. The total number were 63 samples for each exon. Blood samples were collected from Libyan patients (Department of Paediatric Hematology, Tripoli Medical Center), and kept in freezer at -20 Cº. The blood samples were transported to DNA laboratory in the Genetic Engineering Department and Human Tissues Department Biotechnology Research Center, Twesha- Libya. Genomic DNA was extracted from whole blood and DNA was visualized by Agarose Gel Electrophoresis, and DNA concentration was measured by Spectrophotometer. DNA was amplified by polymerase chain reaction (PCR) technique. Mutations were identified by restriction enzymes Taq I and Xba I. Taq I was used to detect nonsense mutations in Exon 23, 2147 [ Arg Stop codon (Term)], Exon 24, 2209 [ Arg Stop codon (Term)], and Exon 26 A+B, 2307 [ Arg Stop codon (Term)]. Our result showed no mutations were detected by using restriction enzyme Taq I in Exons 23, codon 2147, Exon 24, codon 2209, and Exon 26A+B, codon 2307. But by using restriction enzyme Xba I, we found six insertion mutations in Exon 26A, 3919 (ins GA) 11.3 % of 53 patients, and two partial deletion mutations in Exon 23 (3.8 % of 53 patients) refer to area failed to be amplified by PCR.
نادية نور الدين الصربوط (2010)

سرطان الثدي من أكثر الأمراض خبثا، ويعتبر المسبب الأساسي للوفاة بين النساء بعد سرطان الرئة. وهو من أكثر الأمراض انتشارا بين النساء بعد سرطان الجلد. والهدف من هذه الدراسة هو التعرف على احتمال وجود الطفرتين 185delAG و5382insCلمورثة سرطان الثدي1 (BRCA1) بين 77 سيدة ليبية من اللواتي يترددن على عيادة الثدي-بقسم الجراحة – المستشفى المركزي-طرابلس – ليبيا. والحالات المختارة كانت: 32 حالة مشخصة بسرطان الثدي و8 حالات من المصابات بأمراض حميدة بالثدي , 24 حالة من أقارب المريضات بسرطان الثدي، و13 سيدة غير مصابة بسرطان الثدي كمجموعة السيطرة. تم سحب خمسة مليليتر من الدم من كل امرأة ، وجمعت في أنابيب تحتوي على مادة مانعة للتجلط الدم (EDTA) وحفظت في المجمد في (-20ºC) ، نقلت عينات الدم إلى معامل الأحماض النووية لقسم الأنسجة البشرية وقسم الهندسة الوراثية – مركز بحوث التقنيات الحيوية – طويشة – ليبيا ، لأجل دراسة الحمض النووي ، باستخدام سلسلة تفاعل إنزيم متعدد البلمرة (Polymerase Chain Reaction (PCR)) لكشف الطفرات بواسطة سلسلة تفاعل إنزيم متعدد البلمرة مفصولة الطفرات )((MS-PCR Mutagenically Separated PCR) لتضخيم exons الطبيعية و exons المطفرة وذلك بواسطة بوادئ ((Primers خاصة لتشخيص الطفرات , إذا عُثر على الطفرة في أحد exons سيكون هناك حزمتان ((band باستخدام الترحيل الكهربي الهلامي (Agarose gel Electrophoresis) لاكتشاف الطفرات في exons2 و20. نحن وُصِلنَا إلى الهدفِ في هذه الدراسةِ، لمعْرِفة الوجودِ أَو عدمِ الوجود لطفراتْ delAG185 و5382insC في مورثة BRCA1 بين الإناث الليبياتِ، اللواتي يترددن على عيادةَ الثدي-قسم الجراحةِ-مستشفى المركزي-طرابلس-ليبيا؛ كَانَ كالتّالي: -الطفرة 185delAG كَانَت موجودة في 52 من أصل 77 (67.5 %) مِنْ العينات المدروسة، والطفرة 5382insC كَانَت غائبَة في هذه الدراسةِ، حيث العينات لَها حزمة واحدة (تكون حزمة طبيعيةَ) بينما العيناتَ لَها حزمتان (تكون حزمة واحدة طبيعيةَ والحزمة الأخرى غير طبيعية). وفي الخلاصة، نَتائِجنا كانت مشابهة لنَتائِجِ الدِراساتِ الأخرى، حيث تُساعدُ معرفةَ وجودِ الطفرات في التشخيصِ المبكّرِ وهذه تَزِيدُ معدل بقاء على قيد الحياة للنِساءِ للمصابات بسرطان الثدي. Abstract Breast cancer is more the malignancies diseases, and is the primary cause of death among women after lung cancer; is the most spread diseases among women with the exception of skin cancer. The aim of this study is to identify the presence of mutations 185delAG and 5382insC for BRCA1gene in 77 Libyan females attending the breast clinic, Surgery Department, Central Hospital, Tripoli - Libya. The selected cases were: 32 cases diagnosed with breast cancer and 8 cases of women with benign breast disease, 24 cases of relatives of patients with breast cancer, and 13 women without breast cancer as control. Five milliliters of blood were drawn from each woman, and collected in tubes containing EDTA anticoagulant and kept in freezer (-20ºC). The blood samples were transported to DNA laboratory for the Human's Tissues Department and the Genetic Engineering Department- Biotechnology Research Centre-Twesha - Libya, for DNA study; using Polymerase Chain Reaction (PCR); to detect mutations by Mutagenically Separated PCR (MS -PCR), to amplify the normal exons and mutant exons by specific primers for diagnosis of these mutations; if the mutation was found in one of the exons; there will be two bands by use Agarose gel Electrophoresis to detect mutations in exons 2 and 20.We have been reached to the aim this study, for knowing the existence or non-existence of two mutations,185delAG and 5382insC in BRCA1 gene among Libyan females attending the breast clinic, Surgery Department, Central Hospital, Tripoli-Libya; were as follows:- the 185delAG mutation was present in 52 out of 77 (67.5%) of studied samples, and 5382insC mutation was absent in this study, where samples have one band (are normal band) while samples have two bands ( are normal band & another band abnormal). In conclusion, our results were similar to the results of other studies, where knowledge of the existence of mutations helps in early diagnosis and this increases the survival rate for women with breast cancer.
أمل عبد السلام الصربوط (2011)

Abstract Biological studies of Nezara viridula (L.) (Heteroptera:Pentatomidae) and its parasitoid Trissolcus basalis (Wollaston)(Scelionidae: Hymenoptera) collected from Libya were conducted in a rearing room at 25 ± 5°C, 65 ± 10 % R.H. and 16:8 LD photoperiod.During the course of rearing of N. viridula the eggs incubation periodvaried between 4-7 days. The newly emerged nymphs and the 5th stage had the highest mortality in comparison with 1st, 2nd, 3rd and 4thstages .studies on T. basalis included: SEM, development time, parasitoid mating behavior and oviposition behavior, functionalresponse and fecundity. The development time was 4 to 10 days underthe laboratory condition. Adult males lived longer than females.Mating time took from 12.2 to 19.5 seconds. In case of ovipositionbehavior serial of behaviors were observed and recorded. The egg selection period was 0: 00: 08 - 0: 00: 25 min, oviposition period was 0: 01: 04 - 0: 01: 41 min. Egg marking period was 0: 00: 11 - 0: 00: 25 min. The longest period was the oviposition period followed by egg marking period followed by egg selection period.In functional response study was found a highly positive correlation ( r = 0.974 ) number of egg masses exposed and the number of parasitoid emerged. Fecundity experiments showed that the daily ii production of progeny by female of T. basalis during 6-10 dayslifetime showed that female progeny production was higher than male progeny production. The maximum period offspring production was 6-10 days. There was a highly negative correlation ( r = - 0.900 ) between the number of eggs parasitized and parasitoid age . Also there is a highly signifcant difference between the fecundity of T. basalis newly emerged of 24 h and after 7 days after emergence from N. viridula eggs. The number of mature eggs in the ovary were less in 24h aged female and the fecundity increased over the next 7 days.
عايدة عادل عبد الرحيم بادي (2008)