Physical Properties, Antibacterial and Antioxidant Properties of Raw South Africa Shea Butter against Samples from Libyan Market
Vigorous research has been conducted into the phenology of the shea tree, its usage and that of the shea butter extracted from
the nuts of the shea fruit. Shea butter is the most valuable product in the Shea tree and its use as raw or in cosmetic and pharmaceutical
products was expanded in the last years. The aim was to carry out comparative study between imported South African raw Shea Butter,
one sample from Poland and samples from Libyan market on their physical properties (organoleptic), thin layer chromatography (TLC)
chromatogram, phytochemical screening and diphenylpicrylhydrazil (DPPH) scavenging activity. As well as antimicrobial screening.
Organoleptic test was carried out by comparing color and odor. TLC chromatogram was performed by spotting solution of samples in
hexane on TLC plate and eluted twice in hexane: ethylacetate 8:2 to get good separation. Phytochemical screening was performed to
determine the presence of carbohydrates (Fehling test), steroids, and triterpens (Salkwiski test). DPPH assay was carried out by spraying
TLC sample spots by 0.2% DPPH methanolic solution. Antimicrobial test was conducted on Pseudomonas aeruginosa and Staphylococcus
aureus. Mueller-Hinton agar was used with ciprofolxacin as +ve control and DMSO as–ve control. As a result, Shea Butter samples
showed wide diversity in color and odor which considered normal except two white samples that might undergo refining process, while
spreadability and TLC chromatograms were similar. Samples and control showed presence of carbohydrates, steroids and triterpens.
Control and samples had DPPH scavenging activity. Shea Butter control and samples had no antimicrobial activities against P. aeruginosa
and S. aureus. arabic 17 English 111
Sakina Salem Saadawi, Khairi Ali Alennabi, Hanin Nafed Mughrbi, Kholoud Lotfi, Nesrin Saleh Ali Abdulnabi(4-2020)
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