Effect of Caffeine at Different Concentrations on Behavior and Motor Activity in Mice
Aims: This article aimed to study the effect of different caffeine concentrations on behaviour and
motor activity of mice.
Place and Duration of Study: This study took place in Faculty of Pharmacy, University of Tripoli,
and was conducted between 2017 to 2018.
Methodology: The experiment was carried out using 24 male mice (25-30 gm). Plus maze was
used for screening antianxiety effect of caffeine. While swimming maze was used to test the
antidepressant effect. Descriptive statistics was performed using SPSS (version 22), followed by
one sample Kolmogorov-Simirnov test. One-Way ANOVA was applied to compare between groups
and Post Hoc test (LSD).
Results: At a dose of 100 mg/kg, caffeine produce significant decrease in the duration of immobility
using forced swimming maze; while the lower (25 mg/kg) and the higher (200 mg/kg) doses did not
produce any changes compared to the control. In plus maze, Caffeine decreases the anxiety measure at the dose used of 100 mg/kg; but did not change the anxiety measure when lower (25
mg/kg) or higher (200 mg/kg) doses used compared to the control. The spontaneous motor activity
was decreased significantly after administration of the higher dose of 200 mg/kg; the lower dose (25
mg/kg) showed insignificant increase, while the dose of 100 mg/kg produce insignificant decrease in
the spontaneous motor activity.
Conclusion: Caffeine has dose dependent effect, in a dose 100 mg/kg it produce anxiolytic and
antidepressant like action, while lower (25 mg/kg) and higher (200 mg/kg) doses did not show any
changes. Caffeine also produce dose dependent decrease in the spontaneous motor activity, this
indicate that caffeine produce CNS depression with higher doses. arabic 12 English 73
Sakina S. Saadawi, Khairi A. Alennabi, Sumaya Baayo, Amera Fares, Najwa Alosta, Suher M. Aburawi(3-2020)
