Aim: The aim of the current investigation was to screen the presence of Staphylococci spp., especially S. aureus in meat, meat products of different animal species, and some seafood sold in some retail markets in Libya using cultural and molecular techniques, and to study their antibiotics resistance profiles. Materials and Methods: A total of 139 samples from red meat, meat products, and seafood were collected from many areas in Libya. Enumeration and isolation of Staphylococci spp. and S. aureus by normal cultural methods followed by molecular identification using molecular techniques by bacterial DNA extraction and partial sequencing of 16S rDNA. Results: Out of 139 samples, 112 (80.6%) were contaminated with different species of Staphylococci based on cultural characteristics of Staphylococci on Baird-Parker medium, for which S. aureus was detected in only 32 samples (23%). However, only six out of 18 (33.3%) isolates sent for sequencing were confirmed to be S. aureus using the molecular technique. The six identified isolates of S. aureus were tested for antimicrobial resistance against 24 most commonly used antibiotics. All isolates were resistant to only two antibiotics (cefotaxime and clindamycin). Among these six isolates, only one confirmed to be methicillin-resistant Staphylococcus aureus. Conclusion: Results of this study suggest that food of animal origin could be a source of S. aureus with antimicrobial resistance characteristics that can be spread through the food chain, and raise the importance of these results for public health.
Ibrahim Eldaghayes(6-2019)

The main objective of this study was to characterize bacteria isolated from seafood, meat and meat products that may cause foodborne illnesses. We plan to use this data to help create a baseline for future research into foodborne illness in Libya. Several food-borne organisms in the genus Vibrio produce a variety of clinical disorders, including septicemia, cholera, and milder types of gastroenteritis. Vibrio cholerae, Vibrio parahemolyticus, and Vibrio vulnificus are among the Vibrio spp. that are usually related with food-borne transmission. A total of 93 samples (Table 1) of seafood, meat and meat products that includes 21 of shrimps; 5 of clam; 20 of fish; 34 samples of raw meat (10 beef, 9 camel meat, 6 mutton and 9 chickens) and 13 samples of meat products (2 beef sausages, 5 beef burgers, 5 chicken burgers and 1 kebab) were randomly collected from different geographic localities in Libya [Tripoli, Regdalin (120 km west of Tripoli), Janzour (30 km west of Tripoli) and Tobruk (1400 km east of Tripoli)]. Each sample was 250 g in weight. Out of the 93 cultured samples only 48 (51.6%) yielded colonies on Thiosulfate Citrate Bile Salt agar (TCBS) with culture characteristics of Vibrio spp. More than half (n=27) of processed seafood samples (n=46) yielded colonies on TCBS, while only 44.6% of samples of meat and meat products showed colonies on TCBS. Among cultured seafood samples, the highest bacterial count was recorded in clam with a count of 3.8 х10 4 CFU\g. Chicken burger samples showed the highest bacterial count with 6.5 х10 4 CFU\g. Molecular analysis of the isolates obtained in this study, showed that 11 samples out of 48 (22.9%) were Vibrio spp. Vibrio parahemolyticus was isolated from camel meat for the
Salah M. Azwai(1-2022)

الإلقاء المستمر لمياه الصرف الصحى في المنطقة الساحلية للبحر المتوسط بمدينة طرابلس و التى تمتد من تاجوراء الى جنزور قد ادى الى حوث تأثيرات سلبية مستمرة على كل من الأحياء المائية و المستهلك بصورة اصبحت ظاهر في تلك المنطقة الساحلية. لقد عرف التلوث الكيميائي المرتبط بتركيبة الصرف الصحى بتأثيره الشديد على جهاز المناعة في الاسماك مما يؤدى الى زيادة في قباليتها للإصابة بالبكتيريا المرضية. في الدراسة الحالية تم عزل أربعة عترات من الميكروب السبحى "ستربتوكوكس" من أسماك المرجان العادى التى تم تجميعها من المنطقة الساحلية لشاطئ طرابلس في المنطقة الممتدة من تاجوراء الى جنزور. توضح النتائج ان ميكروب ستربتوكوكس ايناى (Streptococcus iniae) كان الأكثر شيوعا فى فصلى الصيف و الربيع يليهم على الترتيب ميكروب ستربتوكوكس ديسجلاكتيا (Streptococcus dysgalactiae) أما ميكروب ستربتوكوس فوكى (Streptococcus phocae) فقد كان الأكثر شيوعا في موسم الشتاء. Abstract: The continuous dumping of municipal sewage into the coastal zone of the Mediterranean Sea has been reported to have greater negative impacts on both aquatic animals as well as human consumer. The chemical pollutants in the sewage composites would have deleterious damaging effects on the fish immune system with consequent vulnerability to bacterial invasions. In the current study, four Streptococcal bacterial species were successfully isolated from the Common Pandora fish (Pagellus erythrinus) collected from the coastal zone of Tripoli through the area from Tajourah to Janzour localities. Strept iniae was the most prevalent Streptococci in examined fish during summer and spring seasons followed by Strept dysgalactiae then Enterococus fecalis. Streptococcus phocae was the most prevalently isolated Streptococci from examined fish during the winter season, while Enterococcus fecalis was recorded to be the second on the list.
طارق دخيل حسن (2015)