أجريت هذه الدراسة بهدف تقييم فاعلية مستخلص نبات القرضاب علي عملية تقليل حمض البوليك في الدم و بالتالي منع ترسيبه في المفاصل و الكلى و الأطراف. تم إستخدام مستخلص جذور نبات القرضاب كعشبة طبية لعلاج النقس و الروماتيزم و اليرقان، و التهاب الشعب الهوائية، و الحمى، الطفح الجلدي و الجروح و فقا للابحيات و المراجع السابقة. في هذه الرسالة، تم تقسيم عدد خمسة و ستون فأر ابيض (ذكور) بشكل عشوائي إلى ست مجموعات. حيث تم إحداث زيادة حمض البوليك في الفئران باستعمال اوكزانات البوتاسيوم المذابة في مادة الصوديوم كربو كسي ميثايل سيلييلوز بنسبة (0.5%) وتم حقنها في الفئران عن طريق الغشاء البريتولى، ومن بعد ذلك معالجتها بمستخلص النبات عن طريق الفم بجرعة محددة حسب المراجع العلمية السابقة وتم مقارنة النتائج العلاجية باستخدام مستخلص النبات بتلك المتحصل عليها باستخدام أدوية كيميائية "مثل" الوبيورينول" لمعرفة فاعلية مستخلص النبات كمضاد لزيادة حامض البوليك و كانت هناك مجموعة ضابطة دون أخد علاج. تم متابعى آلية العلاج عن طريق سحب عينات الدم و الذبح لفترات مختلفة و قراءة المتغيرات في كل فترة و تم التقييم عن طريق التغيرات البيوكيميائية (قياس و ظائف الكلي، وو ظائف الكبد، و تحديد كمية حمض البوليك في الدم و كذلك تحديد كمية البروتين و الكرياتنين في البول خلال 24 ساعة). هملت التجربة دراسة نسيجية باستخدام المجهر الضوئي لمتابعة التغيرات في الكبد و الكلى في المجموعات المختلفة. أثبتتي تخفيض نسبة حمض البوليك للفئران المست الدراسة نجاح مستخلص نبات القرضاب يعتبر افضل من حيث النتائج المتحصل عليها كيميائيا و نسبيجيا من تلك المتحصل عليها باستخدلم الدواء "الكيميائي التقليديىى الوبيورينول". و أخيرا أشارت نتائجنا إلى أن مستخلص جذور نبات القرضب يعتبر ذو فاعلية للوقاية و العلاج الاثارالسلبية على زيادة حمض البوليك في الدم. Abstract: The present study was carried out to investigate the in vivo hypouricemic activity of butanol extract of the roots of Polygonum equisetiforme against Oxonate -induced hyperuricemia in male albino Wister rats. The roots of this species are used in traditional medicinal system for the treatment of gout, rheumatism, jaundice, bronchitis, fever, skin eruptions and wounds. In this study, sixty five male albino rats were divided randomly into six groups. Intraperitoneal (i.p) administration of Potassium Oxonate (250 mg/kg b.w), an uricase inhibitor was used to induce hyperuricemia and given one hour before administration of the tested compound, for 14 consecutive days in groups 2, 5 and 6. The butanol extract of Polygonum equisetiforme (66.82 mg/kg b.w) was orally administered for 14 consecutive days in groups 3 and 5. Allopurinol (10 mg/kg.b.w) was used as the positive control, and given orally for 14 consecutive days in groups 4 and6. At the end of the study, rats were sacrificed under diethyl ether general anesthesia. Serum level of uric acid, liver and kidney functions were measuredcolorometrically and enzymatically. The results showed that Polygonumequisetiforme extract has a potent hypouricemic activity (group 5). While the hypouricemic action of allopurinol has been shown to be of comparatively lesser activity than that of P. equisetiforme (group 6), Moreover allopurinol showedsome adverse effects such as hepatitis and nephropathy. Histopathological changes of kidney and liver were also correlated with the recorded biochemical results. Finally our results indicated that P. equisetiforme roots extract may be effective for the prevention and treatment of hyperuricemia.
سميرة أحمد شلايك (2015)

Aim: The aim of this work was to isolate and molecularly identify enterohemorrhagic Escherichia coli (EHEC) O157 in milk and dairy products in Libya, in addition; to clear the accuracy of cultural and biochemical identification as compared with molecular identification by partial sequencing of 16S rDNA for the existing isolates. Materials and Methods: A total of 108 samples of raw milk (cow, she-camel, and goat) and locally made dairy products (fermented cow’s milk, Maasora, Ricotta and ice cream) were collected from some regions (Janzour, Tripoli, Kremiya, Tajoura and Tobruk) in Libya. Samples were subjected to microbiological analysis for isolation of E. coli that was detected by conventional cultural and molecular method using polymerase chain reaction and partial sequencing of 16S rDNA. Results: Out of 108 samples, only 27 isolates were found to be EHEC O157 based on their cultural characteristics (Tellurite-Cefixime-Sorbitol MacConkey) that include 3 isolates from cow’s milk (11%), 3 isolates from she-camel’s milk (11%), two isolates from goat’s milk (7.4%) and 7 isolates from fermented raw milk samples (26%), isolates from fresh locally made soft cheeses (Maasora and Ricotta) were 9 (33%) and 3 (11%), respectively, while none of the ice cream samples revealed any growth. However, out of these 27 isolates, only 11 were confirmed to be E. coli by partial sequencing of 16S rDNA and E. coli O157 Latex agglutination test. Phylogenetic analysis revealed that majority of local E. coli isolates were related to E. coli O157:H7 FRIK944 strain. Conclusion: These results can be used for further studies on EHEC O157 as an emerging foodborne pathogen and its role in human infection in Libya.
Salah M. Azwai(1-2016)

We conducted a cross-sectional study during 2013 to quantify the serological prevalence of peste des petits ruminants (PPR) infection and to investigate host factors associated with PPR infection in small ruminants in Libya. A two-stage sampling design was carried out. A total number of 148 flocks owning at least 100 heads each were randomly selected. Sixteen to forty-eight samples were collected from each selected flock. A total number of 3,508 serum samples from unvaccinated animals were collected and analysed at IZSLER Brescia, Italy, by using competitive ELISA, IDvet innovative diagnostics (IDvet 310, France). The overall serological prevalence among SR was 33% (95% CI: 31.4–34.5). Significant differences between the prevalence in the geographical branches were observed. The lowest prevalence level was observed in Zawiyah branch (16.1%), whereas the highest value was obtained for the Sabha branch (56.8%). Considering the age, a serological prevalence of 24.7%, 31.5% and 42.1% was observed in SR
Abdulwahab Kammon, A. Dayhum, M. Sharif, I. Eldaghayes, P. Calistri, M. L. Danzetta, D. Di Sabatino , A. Petrini, G. Ferrari, S. Grazioli, G. Pezzoni, E. Brocchi(3-2017)