Opinion Cough in kids less than 6yrs old whether being with sputum i.e. wet or without i.e. dry and parental asking about any medicine stopping this symptom certainly if being dry i.e. irritating and disturbing sleep. So most studies being done on this subject proved the following: a) It is not wise to suppress cough because it is natural defense mechanism to expel infected mucus i.e. sputum out of the body and clearing the airways to improve oxygenation so never to prescribe antitussive i.e. cough suppressant. b) sputum mucolytic agents and there are many agents their purpose to liquify it and get it watery to be easy expectorated again studies proved that the best muculytic agent is Good Hydration so no need to use except where there is a mucus retention in the lungs like case of brocheictasis. c) WHO recommendation made about 6 yrs back was never to prescribe any cough medicine whether antitussive or mucolytic to kids less than 6 yrs old. Myself and since about 10 years I had not prescribed any cough suppressant to children despite of age but if kids older than 6 years old I do prescribe mucolytic agent made certainly for kids like amydramine syrup which contains antihistamine diphenhydramine and without restrictions. In kids less than 6yrs old again I do prescribe mucolytic made for kids like soolan or Amydramine pediatric syrup in trial to hit 2 birds which are sedating and antihistamine effects and satisfying anxious parents and it does work almost in all cases arabic 6 English 32
Hisham Mukhtar Alhaashimi Alrabty(5-2017)

Efficient extraction of genomic DNA (gDNA) from biological materials found in harsh environments is the first step for successful forensic DNA profiling. This study aimed to evaluate two methods for DNA recovery from animal tissues (livers, muscles), focusing on the best storage temperature for DNA yield in term of quality, quantity, and integrity for use in several downstream molecular techniques. Six male Swiss albino mice were sacrificed, liver and muscle tissues (n=32) were then harvested and stored for one week in different temperatures, -20C, 4C, 25C and 40C. The conditioned animal tissues were used for DNA extraction by Chelex-100 method or NucleoSpin Blood and Tissue kit. The extracted gDNA was visualized on 1.5% agarose gel electrophoresis to determine the quality of gDNA and analysed spectrophotometrically to determine the DNA concentration and the purity. Both methods, Chelex-100 and NucleoSpin Blood and Tissue kit found to be appropriate for yielding high quantity of gDNA, with the Chelex100 method yielding a greater quantity (P < 0.045) than the kit. At -20C, 4C, and 25C temperatures, the concentration of DNA yield was numerically lower than at 40C. The NucleoSpin Blood and Tissue kit produced a higher (P=0.031) purity product than the Chelex-100 method, particularly for muscle tissues. The Chelex-100 method is cheap, fast, effective, and is a crucial tool for yielding DNA from animal tissues (livers, muscles) exposed to harsh environment with little limitations.
Huda H. Al-Griw, Zena A. Zraba, Salsabiel K. Al-Muntaser, Marwan M. Draid, Aisha M. Zaidi, Refaat M. Tabagh , Mohamed A. Al-Griw(8-2017)

Exposure to chemicals has been shown to adversely affect CNS health in rodents and humans. The objective was to evaluate, in-vivo, the effects of trichloroethane (TCE), a ubiquitous environmental contaminant, on the integrity of neural cells. A group of albino mice was injected intraperitoneally twice weekly for three weeks with TCE (100 and 400 µg/kg). Animals were followed up for signs of toxicity and death. Alterations in neural tissues have also been investigated by histopathology The results showed a large number of degenerative neural cells (pyknosis of nuclei, DNA fragmentation, chromatin condensation) in the 100 and 400 µg/kg TCE-treated groups comparing to controls. Although there were no significant effect on the neural cell counts, the pattern of increased degenerative cells in TCE-treated groups was higher compared to controls. The results also showed that TCE led to a significant increase in the percent of degenerative neurons. There was also a significant reduction in the percent of neurons. These results correlated with the increase in the percent of glia. This study indicates that TCE exposure had detrimental impact on neural cells, and that neurons are more vulnerable to TCE than glia in this in-vivo mouse model.
Mohamed A. Al-Griw, Naser M. Salama, Soad A. Treesh, Lubna N. Algadi, Abdul hakim Elnfati(7-2015)