Evidence based medicine, clinical reasoning, self-directed critical thinking and problem solving approach are mandatory in order to acquire better retained and usable knowledge in a clinical context through student-centered teaching, and team interpersonal skills promotion. Adoption of new and high standards methods of teaching such as 3D models [24,25], along with updated responsive teaching materials are mandatory and represent pre-requirements for accredited medical schools
Aisha Nasef, Mohamed A Al-Griw, Adel El Taguri(5-2020)

Efficient extraction of genomic DNA (gDNA) from biological materials found in harsh environments is the first step for successful forensic DNA profiling. This study aimed to evaluate two methods for DNA recovery from animal tissues (livers, muscles), focusing on the best storage temperature for DNA yield in term of quality, quantity, and integrity for use in several downstream molecular techniques. Six male Swiss albino mice were sacrificed, liver and muscle tissues (n=32) were then harvested and stored for one week in different temperatures, -20C, 4C, 25C and 40C. The conditioned animal tissues were used for DNA extraction by Chelex-100 method or NucleoSpin Blood and Tissue kit. The extracted gDNA was visualized on 1.5% agarose gel electrophoresis to determine the quality of gDNA and analysed spectrophotometrically to determine the DNA concentration and the purity. Both methods, Chelex-100 and NucleoSpin Blood and Tissue kit found to be appropriate for yielding high quantity of gDNA, with the Chelex100 method yielding a greater quantity (P < 0.045) than the kit. At -20C, 4C, and 25C temperatures, the concentration of DNA yield was numerically lower than at 40C. The NucleoSpin Blood and Tissue kit produced a higher (P=0.031) purity product than the Chelex-100 method, particularly for muscle tissues. The Chelex-100 method is cheap, fast, effective, and is a crucial tool for yielding DNA from animal tissues (livers, muscles) exposed to harsh environment with little limitations.
Huda H. Al-Griw, Zena A. Zraba, Salsabiel K. Al-Muntaser, Marwan M. Draid, Aisha M. Zaidi, Refaat M. Tabagh , Mohamed A. Al-Griw(8-2017)

Background: Atopic dermatitis (AD) is a common, chronic, relapsing, itchy, skin condition occurring in patients with a personal or family history of atopy, and there is clinical association among different allergic disease in a way that treating one of them will improve the other. Many studies worldwide showed presence of AD in asthmatic children with different prevalence among countries and showed clinical improvement in asthma control on treatment of atopic dermatitis. Therefore, this study was conducted to assess the prevalence of atopic dermatitis among Libyan asthmatic children. Methods: This is an observational cohort study on asthmatic Libyan children who were treated and followed up at Tripoli children hospital in Tripoli, Libya. It carried out on 300 children suffering from asthma admitted from pediatric outpatient department as well as from emergency department and asthma clinic over a period of 24 months; from December 2017 to December 2019. The parents were asked to complete a questionnaire to collect the needed information after their consent being taken. To assure the accuracy and consistency of the methodology (sampling procedure, measurements, and a collection of the data), a standardized protocol was prepared. Data were entered in SPSS statistical package and consequently were analyzed and presented as descriptive statistics. Results: The prevalence of atopic dermatitis among asthmatic Libyan children was 16.7% in our study. The results showed significant relationship between address and prevalence of atopic dermatitis. Conclusion. Further studies are required to address the ethnicity, environmental factors, skin type and others attributed to this problem and we recommend all pediatricians to look for AD in asthmatic children and treat it accordingly arabic 7 English 56
Hisham Alrabty, Munera Addala(5-2020)