المستودع الرقمي لـكلية الطب البيطري

Introduction: A total of 32 young (1-3 months-old) lambs suffered from mortal neurological signs in a period of three months. The lambs were among the offspring of a herd of 300 sheep, in Tarhouna city, Libya. The affected lambs were initially treated with antibiotics, multivitamins and mineral supplements, however, none of these treatments attenuated the death toll among the small lambs. Methods: Three lamb carcasses were submitted to the National Center for Animal Health (NCAH) for post mortem examination. Gross pathological examination of the brains detected hydatid cysts and yellowish lesions within the cerebral cortex. Results: The histopathologic examination of the brain showed the presence of inflammatory lesions and necrotic features surrounded by granulomatous inflammatory patterns, which are accompanied by a few parasitic cysts. Ordinary and modified Ziehl-Neelsen stains for bacterial infection were performed, but the results did not strongly indicate bacterial infection. Toxoplasmosis, particularly T. gondii, and Neospora were first suspected. Immunohistochemistry testing for T. gondii did not show any conclusive indication for the presence of this pathogen. However, meticulous microscopic examination (100x) of the histopathologic features and the patterns of host-pathogen interaction of toxoplasmosis indicate that these lambs may have suffered from necrotic encephalitis associated with a different type of toxoplasmosis.
Abdulwahab Kammon(10-2021)

An experiment was conducted to study chlorpyrifos chronic toxicity in broilers and the protective effect of vitamin C. Oral administration of 0.8 mg/kg body weight (bw) (1/50 LD50) chlorpyrifos (Radar®), produced mild diarrhea and gross lesions comprised of paleness, flaccid consistency and slightly enlargement of liver. Histopathologically, chlorpyrifos produced degenerative changes in various organs. Oral administration of 100 mg/kg bw vitamin C partially ameliorated the degenerative changes in kidney and heart. There was insignificant alteration in biochemical and haematological profiles. It is concluded that supplementation of vitamin C reduced the severity of lesions induced by chronic chlorpyrifos toxicity in broilers.
Abdulwahab Kammon(1-2022)

As attached
Abdulwahab Kammon(9-2010)

Foot-and-mouth disease viruses are often restricted to specific geographical regions and spread to new areas may lead to significant epidemics. Phylogenetic analysis of sequences of the VP1 genome region of recent outbreak viruses from Libya and Saudi Arabia has revealed a lineage, O-Ind-2001, normally found in the Indian subcontinent. This paper describes the characterization of field viruses collected from these cases and provides information about a new real-time RT-PCR assay that can be used to detect viruses from this lineage and discriminate them from other endemic FMD viruses that are co-circulating in North Africa and western Eurasia.
Abdulwahab Kammon(1-2014)

Vibrio vulnificus, a zoonotic bacterial pathogen was isolated for the first time from kidneys of black scorpionfish Scorpaena porcus collected from the south Mediterranean basin (western coast of Tripoli, Libya) during the early summer of 2013. Fish exhibited typical signs of Vibriosis with furuncle like lesions, skin hemorrhages, fin rot and congested spleen. The abdominal cavity, liver, intestinal wall, and gonads were suffering from mild larval nematode infection. Interestingly, a huge portion of the kidney tissue was replaced with the anisakids nematode Anisakis pegreffi. This is considered the first report ofV. vulnificus / A. pegreffi in the Mediterranean black scorpionfish. The identities of the retrieved V. vulnificus isolates were confirmed using morpho-chemical and molecular tools. The research lead us to conclude that the deteriorated nature of the sewage polluted seawater at the western coast of Tripoli could have played a detrimental role in facilitating the invasion with the two diverse etiological agents (V. vulnificus and A.pegreffi) . We also hypothesize that V. vulnificus was lodged within nematode worms or at one of its life stages before invading fish intestine with consequent spread into other internal organs during its visceral larval migration stage.
Abdulwahab Kammon(5-2015)

Geospatial database of farm locations and biosecurity measures are essential to control disease outbreaks. A study was conducted to establish geospatial database on poultry farms in Al-Jabal Al-Gharbi region of Libya, to evaluate the biosecurity level of each farm and to determine the seroprevalence of mycoplasma and its relation to biosecurity level. A field team of 7 Veterinarians belongs to the National Center of Animal Health was assigned for data recording and collection of blood samples. Personal information of the producers, geographical locations, biosecurity measures and description of the poultry farms were recorded. The total number of poultry farms in Al-Jabal Al-Gharbi Region is 461 farms distributed in 13 cities. Out of these, 102 broiler farms and one broiler breeder farm (10 houses) which were in operation during team visit were included in this study. Following collection of blood, sera were separated and tested by Enzyme-linked immunosorbent assay (ELISA) for the presence of antibodies against Mycoplasma (General antigen for M. gallisepticum and M. synoviae). The seroprevalence of Mycoplasma in the region was 28% (29 poultry farms out of 103 were infected). About 50% (23 out of 47) of poultry farms located in Garian city were infected with Mycoplasma and one significant cluster of Mycoplasma infection in the city was identified. Low level of biosecurity was found in poultry farms of the region. Out of the 103 farms included, 63% of poultry houses has a ground of soil and 44% of them has uncoated walls which may influence the proper cleaning and disinfection. Almost 100% of the farms are at risk of exposure to diseases transmitted by wild birds such as avian influenza and Newcastle disease due to absence of wild birds control program. Although, 81% of the farms have entry restrictions, only 20% have disinfectants at entry which increase the risk of exposure to pathogens. The results of this study highlight the weakness points of biosecurity measures in poultry farms of Al-Jabal Al-Gharbi region and high seroprevalence of mycoplasma. Data collected in this study will assist the Veterinary authorities to apply effective disease control strategies.
Abdulwahab Kammon(3-2017)

Infectious bronchitis virus (IBV) is a very dynamic and evolving virus, causing major economic losses to the global poultry industry. Recently, the Libyan poultry industry faced severe outbreak of respiratory distress associated with high mortality and dramatic drop in egg production. Tracheal and cloacal swabs were analyzed for several poultry viruses. IBV was detected using SYBR Green I real-time PCR detection based on the nucleocapsid (N) gene. Sequence analysis of the partial N gene indicated high similarity (~94%) to IBV strain 3382/06 that was isolated from Taiwan. Even though the IBV strain 3382/06 is more similar to that of the Mass type H120, the isolate has been implicated associated with intertypic recombinant of 3 putative parental IBV strains namely H120, Taiwan strain 1171/92 and China strain CK/CH/LDL/97I. Complete sequencing and antigenicity studies of the Libya IBV strains are currently underway to determine the evolution of the virus and its importance in vaccine induced immunity. In this paper we documented for the first time the presence of possibly variant IBV strain from Libya which required dramatic change in vaccination program.
Ibrahim Eldaghayes(12-2013)

A cutaneous melanoacanthoma on the forehead of a 5-year-old male mongrel dog was characterized by the presence of two populations of neoplastic cells: epithelial and melanocytic. The epithelial component consisted of nests of well-differentiated stratified squamous epithelium closely associated with neoplastic melanocytes. According to our survey, three cases of this rare pigmented skin neoplasm of the dog have been published. We present here the histological features of the fourth case of Melanoacanthoma in dogs. This report confirms the benign nature of this tumor and adds to the data that will help determine predilections of age, breed, sex and site of its occurrence.
Ibrahim Eldaghayes(4-2014)