المستودع الرقمي لـجامعة طرابلس

The deleterious effects of the residues of antibacterial drugs have been considered as one of the most serious problems in the world on the basis of their importance in both veterinary and human fields. Therefore, the present study was planned out to evaluate the residual levels of oxytetracycline in camel tissues in Tripoli area in Libya. Forty samples of slaughtered camel's tissues (10 of muscle, 10 of liver, 10 of kidney, and 10 of fat) were collected from different carcasses at different slaughter houses in Tripoli districts. The samples were homogenized, extracted and residual concentrations of oxytetracycline have been measured using liquid chromatography-Mass spectroscopy (LC-MS) technique and oxytetracycline standard. Oxytetracycline residues have been detected in 60% in muscle, 80% in liver, 90% in kidney and 70% in fat samples. The samples, although positive, yet decided acceptable as the detected levels were less than that were regulated by Codex Alimentarius Commission (CAC) for oxytetracycline maximal residual levels (100, 300, 600 and 100 μg/Kg muscle, liver, kidney or fat, respectively). The authors recommended avoiding irrational use of oxytetracycline in veterinary practice and camel in particular; and sticking to the withdrawal time regulated and labelled for drugs used in therapy among veterinary personnel, organizations, and governmental agencies in Libya.
Ibrahim Eldaghayes(9-2015)

This study involved a herd consisting of 200 goats in Green Mountain area, suffering from decrease in weight gain, bloody diarrhoea and severe anaemia and in some cases death within few days. Generally, there was no response to the treatment with antibiotics, anthelmentics and multivitamins. The last animal that died was submitted for post-mortem examination. The disease affecting the herd was diagnosed as coccidiosis. This is the first report of caprine coccidiosis in the Green Mountain Area in Libya.
Ibrahim Eldaghayes(6-2015)

Aim: The aim of this work was to isolate and molecularly identify enterohemorrhagic Escherichia coli (EHEC) O157 in milk and dairy products in Libya, in addition; to clear the accuracy of cultural and biochemical identification as compared with molecular identification by partial sequencing of 16S rDNA for the existing isolates. Materials and Methods: A total of 108 samples of raw milk (cow, she-camel, and goat) and locally made dairy products (fermented cow’s milk, Maasora, Ricotta and ice cream) were collected from some regions (Janzour, Tripoli, Kremiya, Tajoura and Tobruk) in Libya. Samples were subjected to microbiological analysis for isolation of E. coli that was detected by conventional cultural and molecular method using polymerase chain reaction and partial sequencing of 16S rDNA. Results: Out of 108 samples, only 27 isolates were found to be EHEC O157 based on their cultural characteristics (Tellurite-Cefixime-Sorbitol MacConkey) that include 3 isolates from cow’s milk (11%), 3 isolates from she-camel’s milk (11%), two isolates from goat’s milk (7.4%) and 7 isolates from fermented raw milk samples (26%), isolates from fresh locally made soft cheeses (Maasora and Ricotta) were 9 (33%) and 3 (11%), respectively, while none of the ice cream samples revealed any growth. However, out of these 27 isolates, only 11 were confirmed to be E. coli by partial sequencing of 16S rDNA and E. coli O157 Latex agglutination test. Phylogenetic analysis revealed that majority of local E. coli isolates were related to E. coli O157:H7 FRIK944 strain. Conclusion: These results can be used for further studies on EHEC O157 as an emerging foodborne pathogen and its role in human infection in Libya.
Ibrahim Eldaghayes(11-2016)

This study aimed to evaluate the antibacterial activity of flavonoids extracted from two Libyan brown algae namely Cystoseira compressa and Padina pavonica using microwave-assisted extraction method against pathogenic bacteria isolated from meat, meat products, milk and dairy products (Staphylococcus aureus subsp. aureus (5 isolates), Bacillus cereus (3 isolates), Bacillus pumilus (1 isolate), Salmonella enterica subsp. enteric (4 isolates) and Enterohaemor-rhagic Escherichia coli O157 (EHEC O157) (4 isolates)). All of these isolates were muti-drug resistant with high MAR index. The results showed that C. compressa extract exhibited better and stronger antibacterial activities against the seventeen tested isolates with inhibition zones diameter ranged from 14 - 22 mm compared to P. pavonica extract which showed positive effect against 9 isolates with low inhibition zone ranged from 11 - 16.5 mm. Flavonoids extracted from C. compressa also displayed the best spectrum of bactericidal effect with a ratio MBC/MIC ≤ 4 obtained on all susceptible tested bacterial strains. Flavonoids and proanthocyanidins significantly contributed to the antibacterial properties. The mode of action of these active extracts is under investigation.
Ibrahim Eldaghayes(1-2017)

Sporadic outbreaks of foot-and-mouth disease (FMD) have occurred in Libya for almost fifty years. During the spring of 2013, a countrywide serosurvey was undertaken to assess the level of FMD virus circulation and identify FMD virus serotypes in the country. A total of 4221 sera were collected, comprising samples from large ruminants (LR; n=1428 samples from 357 farms) and small ruminants (SR; n=2793 samples from 141 farms). FMD sero-prevalence of NSP antibodies determined by ELISA were 19.0% (271/1428) with 95% CI (16.9 – 21.0) and 13.5% (378/2793) with 95% CI (12.3 – 14.8) for LR and SR samples, respectively. The sero-prevalence of NSP antibodies in LR was 12.3% and 19.8% for age group < 1 year and ≥ 1 year, respectively (X2= 4.95, P= 0.026), while in SR was 3.7%, 13.6% and 21.3% for age group < 1 year, 1-2 year and > 2 year, respectively (X2= 118.1, P= 0.000). These observed NSP serologic profiles support the hypothesis of an endemic level of FMD circulation in Libya. All positive sera were tested for SP antibodies for O, A and SAT-2 FMD virus serotypes. Serotype O was the dominant circulating serotype followed by serotype A, while evidence of SAT-2 was not found. These data provide an insight into the wider epidemiology of FMD in Libya, and contribute to field and laboratory investigations that during 2013 serotype O (O/ME-SA/Ind-2001 lineage) was isolated from clinical samples collected from the country.
Ibrahim Eldaghayes(1-2017)

This study was conducted to investigate the presence of Enterococcus spp. in meat, meat products and seafood. A hundred and four samples were randomly collected from different geographic localities in Libya. The samples were subjected to microbiological analysis for enumeration and isolation of Enterococcus spp. by conventional cultural and molecular identification using PCR and partial sequencing of 16S rDNA techniques. Out of 104 samples, 73 (70.2%) isolates were found to be enterococci based on their cultural characteristics on ESD medium. However, out of 36 samples subjected to molecular identification, only six isolates were confirmed to be Enterococcus spp. using PCR and partial sequencing of 16S rDNA technique. All enterococci strains tested for their antibiotic sensitivity profiles showed high percentage of multi-resistance phenotype. These results can be used for further studies on enterococci as an emerging food borne pathogen and its role in human infection in Libya and would suggest that meat, meat products and seafood might play a role in the spreading of enterococci through the food chain with antimicrobial resistance characteristics.
Ibrahim Eldaghayes(6-2017)

A cross‑sectional study was conducted in Libya in 7 areas of Tripoli to determine the seroprevalence of Peste des Petits Ruminants (PPR) Virus (PPRV) in small ruminants (sheep and goats) between June and August 2013, and to identify the potential risk factors associated with the infection. The study involved 10% of small ruminant herds with ≥ 50 animals in the Tripoli region. They were selected randomly (15 herds), and 35 to 58 samples, depending on its size, were collected from each selected herd. Seven‑hundred and twenty‑one serum samples from unvaccinated animals (601 of sheep and 120 of goats) were collected and then tested using cELISA commercial kit in the National Center of Animal Health Laboratory in Tripoli, Libya. The overall seroprevalence was 46.7% [(sheep 44.3% (266/601) and goats 59.2% (71/120)]. Mean within‑herd prevalence was 48.5% (95% CI: 32.1% ‑ 64.8%), and the herd prevalence was 93.3% (14/15). Various risk factors at the animal and herd levels were analysed by multivariable logistic regression model (forward stepwise). The results identified breed, source of animal, and region as significant risk factors (p < 0.05). As for the source of new animal to the farm, PPRV seroprevalence was highest in illegally imported animals (90.9%), followed by the seroprevalence in animal legitimately acquired (55.8%), and by the seroprevalence in animals belonging to the same herd (4.7%). The seroprevalence among breeds was 69.5% (228/328) in illegally imported animals, whereas 27.7% (109/393) was found to be in local breed. Seroprevalence in the areas considered in this study was higher (66.2%) in Al‑Mashroa area followed by Ein‑zara (57.8%), Arada (50%), Ben‑Own (47%), AL‑Naem (37.5), Ber‑Alalem (24.5) and in Tajora (0%). The results indicated that PPRV virus was actively circulating in Tripoli regions and that the illegal importing of animals was the main source of spreading PPR in Tripoli regions, showing that better efforts should be made to raise public awareness with respect to biosecurity.
Ibrahim Eldaghayes(7-2017)

We conducted a cross-sectional study during 2013 to quantify the serological prevalence of peste des petits ruminants (PPR) infection and to investigate host factors associated with PPR infection in small ruminants in Libya. A two-stage sampling design was carried out. A total number of 148 flocks owning at least 100 heads each were randomly selected. Sixteen to forty-eight samples were collected from each selected flock. A total number of 3,508 serum samples from unvaccinated animals were collected and analysed at IZSLER Brescia, Italy, by using competitive ELISA, IDvet innovative diagnostics (IDvet 310, France). The overall serological prevalence among SR was 33% (95% CI: 31.4–34.5). Significant differences between the prevalence in the geographical branches were observed. The lowest prevalence level was observed in Zawiyah branch (16.1%), whereas the highest value was obtained for the Sabha branch (56.8%). Considering the age, a serological prevalence of 24.7%, 31.5% and 42.1% was observed in SR
Ibrahim Eldaghayes(7-2017)